Comparison between miR-199b-5p and AFP in the diagnosis of Hepatitis C-associated hepatocellular carcinoma
Osama, N., Amer, N., Mohamed, A., Salah, M., Ezz Al Arab, M., El-Awady, R. (2025). Comparison between miR-199b-5p and AFP in the diagnosis of Hepatitis C-associated hepatocellular carcinoma. EKB Journal Management System, 5(1), 99-108. doi: 10.21608/aijpms.2024.214335.1219
Noha Osama; Noha N Amer; Amal A Mohamed; Mohamed Salah; Mohamed R Ezz Al Arab; Rehab R El-Awady. "Comparison between miR-199b-5p and AFP in the diagnosis of Hepatitis C-associated hepatocellular carcinoma". EKB Journal Management System, 5, 1, 2025, 99-108. doi: 10.21608/aijpms.2024.214335.1219
Osama, N., Amer, N., Mohamed, A., Salah, M., Ezz Al Arab, M., El-Awady, R. (2025). 'Comparison between miR-199b-5p and AFP in the diagnosis of Hepatitis C-associated hepatocellular carcinoma', EKB Journal Management System, 5(1), pp. 99-108. doi: 10.21608/aijpms.2024.214335.1219
Osama, N., Amer, N., Mohamed, A., Salah, M., Ezz Al Arab, M., El-Awady, R. Comparison between miR-199b-5p and AFP in the diagnosis of Hepatitis C-associated hepatocellular carcinoma. EKB Journal Management System, 2025; 5(1): 99-108. doi: 10.21608/aijpms.2024.214335.1219

Comparison between miR-199b-5p and AFP in the diagnosis of Hepatitis C-associated hepatocellular carcinoma

Azhar International Journal of Pharmaceutical and Medical Sciences
Article 8, Volume 5, Issue 1, January 2025, Page 99-108  XML PDF (464.77 K)
Document Type: Original research articles
DOI: 10.21608/aijpms.2024.214335.1219
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Authors
Noha Osama email 1; Noha N Amer2; Amal A Mohamed3; Mohamed Salah4; Mohamed R Ezz Al Arab5; Rehab R El-Awady2
1Pediatritic Nutrition, Fitoverfat Nutrition Clinic
2Department of Biochemistry and Molecular Biology, Faculty of pharmacy (Girls) Al-Azhar University Cairo, Egypt
3Department of Biochemistry and Molecular Biology, National Hepatology, and Tropical Medicine Research Institute
4Department of Internal Medicine, National Hepatology and Tropical Medicine Research Institute, Cairo, Egypt
5Department of Hepatology, Ahmed Maher Teaching Hospital, Egypt
Abstract
Background: Hepatocellular carcinoma (HCC) is a global burden. Early detection through surveillance methods has increased patient survival. The miR-199b-5p could be a powerful biomarker for HCC detection. Therefore, this study aimed to estimate the diagnostic value of miR-199b-5p as an accurate non-invasive diagnostic marker for early diagnosis of hepatitis C virus (HCV)-induced HCC compared to Alpha-fetoprotein (AFP).
Patients and Methods: 300 participants were enrolled in this study and categorized into three main groups:100 (HCV), 100 (HCC), and 100 healthy controls. The expression profile of miRNA-199b-5p was analyzed using Real-time polymerase chain reaction (real-time PCR) technology for all participants enrolled in this study.
Results: miRNA-199b-5p had more diagnostic performance in differentiating the HCC group from the control group, showing 72% sensitivity and 62% specificity (AUC:0.770) in comparison to AFP, which had less significant diagnostic performance in differentiating the HCC group from the control group. Showing 67% sensitivity and 51% specificity (AUC:0.675).
Conclusion: The results indicated a higher diagnostic value of miRNA-199b-5p than AFP in detecting HCC patients. Thus, miRNA-199b-5p could be a diagnostic biomarker for HCC with superiority over AFP.
Keywords
HCC; HCV; miRna-199b-5p; AFP
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