Molecular differentiation and phylogenetic analysis of the Egyptian foot-and-mouth disease virus SAT2 Collected from Ismailia Governorate in Egypt | ||||
| Journal of Current Veterinary Research | ||||
| Article 3, Volume 10, Issue 1, 2016, Page 21-28 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jcvr.2016.37878 | ||||
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| Authors | ||||
Mohamed Mahmoud  1; Fawzy M.2
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| 1Department of Animal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt. | ||||
| 2Department of virology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt. | ||||
| Abstract | ||||
| Foot-and-mouth Disease (FMD) is a serious contagious viral disease affecting cloven hoofed animals. Egypt is endemic by three FMDV serotypes, serotype O, A and SAT2. The aims of this study were to compare between virus isolation (VI) and real time RT-PCR (rRT-PCR) in diagnosis of FMDV. Furthermore, detection and molecular characterization of FMDV serotype SAT2 in clinically affected cattle in Ismailia governorate, Egypt during December 2015. A total of twenty oral epithelial tissues were collected and tested by virus Isolation (VI) Real time polymerase chain reaction (rRT-PCR) was applied for FMDV serotyping. All the tested twenty tissue samples were positive by rRT-PCR. However, only 17 (85%) samples were successfully positive by VI that confirmed by rRT-PCR. All the positive samples by rRT-PCR were subjected to RT-PCR for detection of FMDV serotype SAT2 and amplification of VP1 region for further characterization. Out of 20 samples, 11 samples (55%) were positive and further phylogenetic analysis revealed circulation of a single strain of FMDV serotype SAT2, topotype VII. This strain was closely related to the previously detected strains in Egypt 2012 outbreak with percentage of identity ranged from 99.1% to 99.8%. In conclusion, rRT-PCR is highly sensitive and specific assay for diagnosis of FMDV. In addition, FMDV serotype SAT2 serotype VII which still circulating and causing clinical disease in cattle population in Ismailia governorate. Keywords: FMD, virus isolation, rRT-PCR, FMDV serotypes SAT-2, Ismailia-Egypt | ||||
| Keywords | ||||
| FMD; virus isolation; rRT-PCR; FMDV serotypes SAT-2; Ismailia-Egypt | ||||
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