Antidiabetic Activity and GC-MS Analysis of n-Hexane Leaf Extract of Codiaeum variegatum (Euphorbiaceae) | ||||
| Azhar International Journal of Pharmaceutical and Medical Sciences | ||||
| Article 11, Volume 5, Issue 1, January 2025, Page 128-140 PDF (509.81 K) | ||||
| Document Type: Original research articles | ||||
| DOI: 10.21608/aijpms.2024.264259.1250 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Samar S Tolba  1; Hala Sh Mohammed 2; Mosad Ghareeb3; Abd El-Salam Mohamed4
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| 1National Food Safety Authority food safety inspector | ||||
| 2Department of Pharmacognosy and Medicinal Plants, Faculty of Pharmacy (Girls), Al Azhar University, Cairo 11754, Egypt | ||||
| 3Department of Medicinal Chemistry, Theodor Bilharz Research Institute, Giza 12411, Egypt | ||||
| 4Department of Pharmacognosy and Medicinal Plants, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo, Egypt | ||||
| Abstract | ||||
| This study aimed to discover bioactive fatty esters in the n-hexane leaf extract of Codiaeum variegatum (L.) through GC-MS analysis. The extract, known for its antidiabetic properties, was subjected to in vitro antidiabetic activity tests using α-Glucosidase and α-Amylase assays. The GC-MS analysis identified 55 potential therapeutic compounds. The main phytoconstituents comprised; Hexadecanoic acid, methyl ester (6.12%), n-hexadecanoic acid (3.42%), Hexadecanoic acid, methyl ester (6.12%), n-Hexadecanoic acid (3.42%), Hexadecanoic acid, trimethylsilyl ester (2.11%), Linoleic acid, hydroxy methyl ester (7.01%), Linoleic acid, methyl ester (20.67%), Phytol (1.63%), Methyl stearate (4.65%), Cyclopropane- octanoic acid, 2-[[2-[(2-Ethyl cyclopropyl) methyl]cyclopropyl]methyl], methyl ester (7.91%), Octadecanoic acid (1.54%), 9-Octadecenoic acid, 12-hydroxy-methyl ester, [R-(Z)] (10.89%), 7,10-Octadecadienoic acid, methyl ester (1.98%), cis-5,8,11-Eicosatrienoic acid, methyl ester (3.71%), Delta cuprenene (1.37%), 2H-Pyran-2-one, tetrahydro-6-tridecyl-(1.37%) and Bis(2-ethylhexyl) phthalate (5.63%). The extract showed weak to moderate inhibition on α-Glucosidase and α-Amylase with IC50 values of 27.40 and 24.43 µg/ml, respectively, compared to Acarbose. More n-hexane extract is required for similar inhibition, indicating Acarbose’s higher potency. This suggests C. variegatum (L.) has diverse bioactive chemicals. While these findings are significant for therapy development, further research is needed to understand potential applications and risks. This study opens avenues for future research into bioactive compounds and their therapeutic potential. | ||||
| Keywords | ||||
| Codiaeum variegatum; n-hexane extract; GC-MS; Fatty esters; Antidiabetic | ||||
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