Characterization of brucella strains recovered from slaughtered cows | ||||
| Journal of Current Veterinary Research | ||||
| Article 4, Volume 10, Issue 1, 2016, Page 29-33 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jcvr.2016.37879 | ||||
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| Authors | ||||
H.I. Hosein  ; R.A. Azzam; S.R. Rouby
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| Department of Veterinary Medicine, Faculty of Veterinary Medicine, Beni-Suef University | ||||
| Abstract | ||||
| Tissue specimens of 22 cows including retropharyngeal, prescapular, prefemoral, internal iliac and supramammary lymph nodes as well as spleen were collected from animals after slaughtering at abattoirs in Beni Suef Governorate. These animals were classified as brucella infected according to test and slaughter policy applied by the veterinary authorities. Blood serum samples were collected at slaughtering and re-examined using Buffered Acidified Plate Agglutination Test (BAPAT) and Rose Bengal Plate Agglutination Test (RBPAT). Tissue specimens were subjected for trials of Brucella isolation, DNA extraction and PCR for detection of Brucella infection. Brucella melitensis biovar 3 was isolated from 12 (54.55%) seropositive cows. Amplification of target gene (Immunodominant antigen, gene bp26) for molecular identification of Brucella in DNA extracts at the genus level showed that the entire DNA extracts of the 22 tissue samples of cows, were all confirmed by the genus-specific PCR as Brucella with amplification of the fragment of 450 bp. | ||||
| Keywords | ||||
| Brucella melitensis biovar 3; PCR | ||||
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