Comparative measurement of Equine influenza virus antibodies by single radial haemolysis and haemagglutination inhibition tests | ||||
| Journal of Current Veterinary Research | ||||
| Article 10, Volume 10, Issue 1, 2016, Page 72-79 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jcvr.2016.37889 | ||||
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| Authors | ||||
| Eman Ebeid; Nehal Saleh* ; Nashwa Madkour | ||||
| Serum and Vaccine Research Institute, Abbasia, Cairo, Postal No: 11381 P.O. Box 13 | ||||
| Abstract | ||||
| Antibodies against Equine influenza (EI) are traditionally quantified by haemagglutination inhibition (HI) or single radial haemolysis (SRH) .Both assays measure antibodies against haemagglutinin. HI test has the advantage of good sensitivity and the results can be obtained within hours. SRH method is of considerable potential value for the serologic diagnosis and surveillance of influenza, as the test performed with undiluted and untreated serum. In order to determine the sensitivity, accuracy and specificity of SRH, 50 serum samples from horses and 15 from G. pigs vaccinated with inactivated equine influenza vaccine were assayed for antibodies by both HI and SRH tests. The proportion of horse serum samples that gave positive SRH reactions and HI were 74 % and samples gave negative result by both tests was 14%.The proportion of symmetry (88%). Serum samples of guinea-pigs with high HI titer gave potent SRH reaction. So it is clear that antibody concentrations specific for equine influenza virus measured using SRH are highly correlated with HI assays and combines the sensitivity of HI test with higher accuracy. | ||||
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