Absence of Caveolin-1 P132L Mutation in Egyptian Breast Cancer Patients | ||||
| Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology | ||||
| Volume 16, Issue 2, December 2024, Page 157-179 PDF (832.85 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/eajbsc.2024.379480 | ||||
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| Authors | ||||
| Mohamed Hosney1; Salwa Sabet1; Mohamed El-Shinawi2; Mona M. Mohamed1 | ||||
| 1Department of Zoology, Faculty of Science, Cairo University, Giza 12613 Egypt. | ||||
| 2Department of General Surgery, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt. | ||||
| Abstract | ||||
| Understanding the distinctive immunomodulatory processes of the breast cancer microenvironment will be crucial for the creation of novel therapeutic approaches. Cav-1's role in BC is still debated because it has been demonstrated to have a dual opposing role, functioning as both an oncogene and a tumor suppressor. Various studies reported the occurrence of a somatic mutation in Cav-1 in patients' primary breast tumors leading to the substitution of a proline amino acid-to-leucine at 132 (P132L) location. Therefore, the current study aims to examine the expression of Cav-1 in tissue samples of patients with breast cancer and assess the incidence of p132L point mutation of the Cav-1 gene in patients with breast cancer. The current study enrolled 50 patients with breast cancer, and the levels of expression of Cav-1 protein were measured by immunohistochemistry. Sequencing of amplified Cav-1 gene in sample groups was done to assess the incidence of a spontaneous mutation (P132L) inside the human Cav-1 in breast cancer patients. The current results demonstrated the absence of P132L target point mutation in all Cav-1 downregulated Egyptian breast cancer patients (90%). Accordingly, the alteration in the expression level of Cav-1 in tissues of Egyptian patients with breast cancer is not due to the P132L target point mutation, but other factors may contribute to this down-expression. | ||||
| Keywords | ||||
| Caveolae; Cav-1; P132L; mutation and tumor suppressor | ||||
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