Altered Serum Levels of Mir-130b-3p & Mir-148a-3p in Egyptian Pediatric Patients with Juvenile Onset Systemic Lupus Erythema‌tosus and Lupus Nephritis
Ashmawy, I., Ramadan, A., Helmy, N., Marzouk, H., Farag, Y., Abd El Aziz, S. (2024). Altered Serum Levels of Mir-130b-3p & Mir-148a-3p in Egyptian Pediatric Patients with Juvenile Onset Systemic Lupus Erythema‌tosus and Lupus Nephritis. EKB Journal Management System, 33(4), 221-226. doi: 10.21608/ejmm.2024.318532.1330
Ingy Ashmawy; Abeer Ramadan; Neveen A. Helmy; Huda Marzouk; Yomna Farag; Shereen H. Abd El Aziz. "Altered Serum Levels of Mir-130b-3p & Mir-148a-3p in Egyptian Pediatric Patients with Juvenile Onset Systemic Lupus Erythema‌tosus and Lupus Nephritis". EKB Journal Management System, 33, 4, 2024, 221-226. doi: 10.21608/ejmm.2024.318532.1330
Ashmawy, I., Ramadan, A., Helmy, N., Marzouk, H., Farag, Y., Abd El Aziz, S. (2024). 'Altered Serum Levels of Mir-130b-3p & Mir-148a-3p in Egyptian Pediatric Patients with Juvenile Onset Systemic Lupus Erythema‌tosus and Lupus Nephritis', EKB Journal Management System, 33(4), pp. 221-226. doi: 10.21608/ejmm.2024.318532.1330
Ashmawy, I., Ramadan, A., Helmy, N., Marzouk, H., Farag, Y., Abd El Aziz, S. Altered Serum Levels of Mir-130b-3p & Mir-148a-3p in Egyptian Pediatric Patients with Juvenile Onset Systemic Lupus Erythema‌tosus and Lupus Nephritis. EKB Journal Management System, 2024; 33(4): 221-226. doi: 10.21608/ejmm.2024.318532.1330

Altered Serum Levels of Mir-130b-3p & Mir-148a-3p in Egyptian Pediatric Patients with Juvenile Onset Systemic Lupus Erythema‌tosus and Lupus Nephritis

Egyptian Journal of Medical Microbiology
Article 27, Volume 33, Issue 4, October 2024, Page 221-226  XML PDF (333.01 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2024.318532.1330
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Authors
Ingy Ashmawy email orcid 1; Abeer Ramadan2; Neveen A. Helmyorcid 1; Huda Marzouk3; Yomna Farag3; Shereen H. Abd El Aziz1
1Department of Clinical and Chemical Pathology, Medical Research and Clinical Studies Institute, National Research Centre, Cairo, Egypt
2Department of Molecular Genetics and Enzymology, Human Genetics and Genome Research Institute, National Research Centre, Cairo, Egypt
3Department of Pediatrics, Faculty of Medicine, Cairo University, Cairo, Egypt
Abstract
Background: Juvenile onset systemic lupus erythematous (JSLE) and the accompanying lupus nephritis (LN) (affects up to 80% of JSLE) harbor more aggressive disease courses and major complications with higher morbidity and mortality risks compared to adult onset SLE. Two microRNAs (miRNAs), miR-130b-3p and miR-148a-3p, are strong candidates in the pathogenesis of both JSLE and LN. Objective: Studying serum expression of miR-130b-3p and miR-148a-3p patients with JSLE and LN to evaluate their contribution to their pathogenesis. Methodology: The study included 100 LN, 50 JSLE and 50 healthy controls (HC), reverse transcriptase real time PCR (RT-QPCR) was used for evaluation of serum levels of miR-130b-3p and miR-148a-3p. Results: miR-130b-3p showed decreased expression in JSLE and LN patients compared to HC and correlated inversely with serum creatinine within patients’ groups. miR-148a-3p significantly decreased expression in JSLE patients compared to controls and LN patients. MiR-148a-3p was elevated in LN cases compared to JSLE but not HC showing inverse correlation with duration of LN and serum creatinine. Positive correlation between miR-130b-3p and miR-148a-3p was detected in diseased groups (r = 0.75, p = 0.001). Conclusion: Our study was the first to analyze both miR-130b-3p & miR-148a-3p expression in JSLE and LN and we concluded that both might affect the pathogenesis of JSLE and that miR-148a-3p may significantly contribute to the pathogenesis of LN among JSLE patients.
Keywords
Keywords: SLE; Juvenile onset systemic lupus erythematous; Lupus nephritis; miR-130b-3p; miR-148a-3p
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