Epigenetic Modifications Among Children with Autism in Response to Phoenix dactylifera: An Intervention Study.
Metwally, A., Mosaad, R., Basha, W., Salah El-Din, E., El-Sonbaty, M., Elkhatib, A., Abouelnaga, M., Yousef, W., Mahmoud, W., Fathy, A., Elshaarawy, G., Shehata, M., Attia, H., Abdel Raouf, E., Elsaied, A., Ashaat, E., Hashish, A., Mehanna, N., Al Swailem, S., Salem, S. (2024). Epigenetic Modifications Among Children with Autism in Response to Phoenix dactylifera: An Intervention Study.. EKB Journal Management System, 67(11), 219-237. doi: 10.21608/ejchem.2024.314174.10234
Ammal M. Metwally; Rehab Mosaad; Walaa A. Basha; Ebtissam M. Salah El-Din; Marwa M. El-Sonbaty; Alshaimaa A. Elkhatib; Marwa W. Abouelnaga; Walaa Yousef; Walaa S. Mahmoud; Asmaa M. Fathy; Ghada A. Elshaarawy; Manal A. Shehata; Hanaa R. M. Attia; Ehab R. Abdel Raouf; Amal Elsaied; Engy A. Ashaat; Adel F. Hashish; Nayra Sh. Mehanna; Saleh M. Al Swailem; Sohair Salem. "Epigenetic Modifications Among Children with Autism in Response to Phoenix dactylifera: An Intervention Study.". EKB Journal Management System, 67, 11, 2024, 219-237. doi: 10.21608/ejchem.2024.314174.10234
Metwally, A., Mosaad, R., Basha, W., Salah El-Din, E., El-Sonbaty, M., Elkhatib, A., Abouelnaga, M., Yousef, W., Mahmoud, W., Fathy, A., Elshaarawy, G., Shehata, M., Attia, H., Abdel Raouf, E., Elsaied, A., Ashaat, E., Hashish, A., Mehanna, N., Al Swailem, S., Salem, S. (2024). 'Epigenetic Modifications Among Children with Autism in Response to Phoenix dactylifera: An Intervention Study.', EKB Journal Management System, 67(11), pp. 219-237. doi: 10.21608/ejchem.2024.314174.10234
Metwally, A., Mosaad, R., Basha, W., Salah El-Din, E., El-Sonbaty, M., Elkhatib, A., Abouelnaga, M., Yousef, W., Mahmoud, W., Fathy, A., Elshaarawy, G., Shehata, M., Attia, H., Abdel Raouf, E., Elsaied, A., Ashaat, E., Hashish, A., Mehanna, N., Al Swailem, S., Salem, S. Epigenetic Modifications Among Children with Autism in Response to Phoenix dactylifera: An Intervention Study.. EKB Journal Management System, 2024; 67(11): 219-237. doi: 10.21608/ejchem.2024.314174.10234

Epigenetic Modifications Among Children with Autism in Response to Phoenix dactylifera: An Intervention Study.

Egyptian Journal of Chemistry
Volume 67, Issue 11, November 2024, Page 219-237  XML PDF (1.89 MB)
Document Type: Original Article
DOI: 10.21608/ejchem.2024.314174.10234
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Authors
Ammal M. Metwally email orcid 1; Rehab Mosaadorcid 2; Walaa A. Bashaorcid 3; Ebtissam M. Salah El-Din4; Marwa M. El-Sonbatyorcid 4; Alshaimaa A. Elkhatiborcid 4; Marwa W. Abouelnaga4; Walaa Youseforcid 3; Walaa S. Mahmoudorcid 3; Asmaa M. Fathy1; Ghada A. Elshaarawyorcid 1; Manal A. Shehataorcid 4; Hanaa R. M. Attiaorcid 5; Ehab R. Abdel Raouforcid 6; Amal Elsaiedorcid 6; Engy A. Ashaatorcid 7; Adel F. Hashishorcid 6; Nayra Sh. Mehannaorcid 8; Saleh M. Al Swailem9; Sohair Salemorcid 2
1Community Medicine Research Department/Medical Research and Clinical Studies Institute/National Research Centre (Affiliation ID: 60014618), Dokki, Cairo, Egypt,
2Molecular Genetics and enzymology Department, Human Genetics and Genome Research Institute, National Research Centre (Affiliation ID: 60014618), Dokki, Cairo, Egypt.
3Biological Anthropology Department/ Medical Research and Clinical Studies Institute/National Research Centre (Affiliation ID: 60014618), Dokki, Cairo, Egypt,
4Child Health Department/ Medical Research and Clinical Studies Institute/National Research Centre (Affiliation ID: 60014618), Dokki, Cairo, Egypt,
5Clinical and Chemical Pathology Department/Medical Research and Clinical Studies Institute, National Research Centre (Affiliation ID: 60014618), Dokki, Cairo, Egypt
6Department of Child with Special Needs/Medical Research and Clinical Studies Institute, National Research Centre (Affiliation ID: 60014618), Dokki, Cairo, Egypt.
7Clinical Genetics Dept./ Human Genetics and Genome Research Institute, National Research Centre (Affiliation ID: 60014618), Dokki, Cairo, Egypt.
8Probiotics Lab, the central laboratory network, Food industries and Nutrition Research Institute, National Research Centre (Affiliation ID: 60014618), Dokki, Cairo, Egypt.
9President of Ethq Agri. Develop. and Land Reclam. co. 33 Abdel Khaleq Tharwat St.Abdeen,Cairo,Egypt
Abstract
This study explored the influence of the regular daily intake of 5 pieces of Ajwa dates fruit (Phoenix dactylifera) for four months on modifications of epigenetic factors related to autism. An interventional exploratory study was conducted on 31 autism-diagnosed children aged 3-12 years. Methylation-specific PCR was used to determine the methylation density of Olfactory receptor family 2 subfamily L member 13 (OR2L13), Reelin (RELN), Proline-Rich Transmembrane Protein 1 (PRRT1), and index of global methylation (Long interspersed nucleotide element-1, LINE-1). Quantitative real-time PCR was used to test the change in the expression of DNA methyltransferase 1 (DNMT1) and chromodomain helicase DNA-binding protein 8 (CHD8), the expression of plasma miR-146A and miR-146B. The study also investigated the clinical, microbial, and biochemical factors' responses to Phoenix dactylifera intake. In response to Ajwa dates fruit intake, a significant decrease in the expression of DNMT1, was shown in 64.5% of children (P=0.012). LINE-1, OR2L13, and PRRT1 showed significant changes in their methylation density. A significant decrease was observed in the pattern of RELN methylation status and the mean expression fold change of MiR-146A for 74.2% and 38.7% of children respectively. Phoenix dactylifera's regular intake plays a role in epigenetic modification among children with autism.
Keywords
Ajwa dates fruit; Autism; epigenetics modulation; DNA methylation, Expression of DNMT1 and CHD8; Expression modulation of plasma miR-146a and miR-146b; Phoenix dactylifera
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