Epigenetic Modifications Among Children with Autism in Response to Phoenix dactylifera: An Intervention Study. | ||
| Egyptian Journal of Chemistry | ||
| Volume 67, Issue 11, November 2024, Pages 219-237 PDF (1.89 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejchem.2024.314174.10234 | ||
| Authors | ||
| Ammal M. Metwally* 1; Rehab Mosaad2; Walaa A. Basha3; Ebtissam M. Salah El-Din4; Marwa M. El-Sonbaty4; Alshaimaa A. Elkhatib4; Marwa W. Abouelnaga4; Walaa Yousef3; Walaa S. Mahmoud3; Asmaa M. Fathy1; Ghada A. Elshaarawy1; Manal A. Shehata4; Hanaa R. M. Attia5; Ehab R. Abdel Raouf6; Amal Elsaied6; Engy A. Ashaat7; Adel F. Hashish6; Nayra Sh. Mehanna8; Saleh M. Al Swailem9; Sohair Salem2 | ||
| 1Community Medicine Research Department/Medical Research and Clinical Studies Institute/National Research Centre (Affiliation ID: 60014618), Dokki, Cairo, Egypt, | ||
| 2Molecular Genetics and enzymology Department, Human Genetics and Genome Research Institute, National Research Centre (Affiliation ID: 60014618), Dokki, Cairo, Egypt. | ||
| 3Biological Anthropology Department/ Medical Research and Clinical Studies Institute/National Research Centre (Affiliation ID: 60014618), Dokki, Cairo, Egypt, | ||
| 4Child Health Department/ Medical Research and Clinical Studies Institute/National Research Centre (Affiliation ID: 60014618), Dokki, Cairo, Egypt, | ||
| 5Clinical and Chemical Pathology Department/Medical Research and Clinical Studies Institute, National Research Centre (Affiliation ID: 60014618), Dokki, Cairo, Egypt | ||
| 6Department of Child with Special Needs/Medical Research and Clinical Studies Institute, National Research Centre (Affiliation ID: 60014618), Dokki, Cairo, Egypt. | ||
| 7Clinical Genetics Dept./ Human Genetics and Genome Research Institute, National Research Centre (Affiliation ID: 60014618), Dokki, Cairo, Egypt. | ||
| 8Probiotics Lab, the central laboratory network, Food industries and Nutrition Research Institute, National Research Centre (Affiliation ID: 60014618), Dokki, Cairo, Egypt. | ||
| 9President of Ethq Agri. Develop. and Land Reclam. co. 33 Abdel Khaleq Tharwat St.Abdeen,Cairo,Egypt | ||
| Abstract | ||
| This study explored the influence of the regular daily intake of 5 pieces of Ajwa dates fruit (Phoenix dactylifera) for four months on modifications of epigenetic factors related to autism. An interventional exploratory study was conducted on 31 autism-diagnosed children aged 3-12 years. Methylation-specific PCR was used to determine the methylation density of Olfactory receptor family 2 subfamily L member 13 (OR2L13), Reelin (RELN), Proline-Rich Transmembrane Protein 1 (PRRT1), and index of global methylation (Long interspersed nucleotide element-1, LINE-1). Quantitative real-time PCR was used to test the change in the expression of DNA methyltransferase 1 (DNMT1) and chromodomain helicase DNA-binding protein 8 (CHD8), the expression of plasma miR-146A and miR-146B. The study also investigated the clinical, microbial, and biochemical factors' responses to Phoenix dactylifera intake. In response to Ajwa dates fruit intake, a significant decrease in the expression of DNMT1, was shown in 64.5% of children (P=0.012). LINE-1, OR2L13, and PRRT1 showed significant changes in their methylation density. A significant decrease was observed in the pattern of RELN methylation status and the mean expression fold change of MiR-146A for 74.2% and 38.7% of children respectively. Phoenix dactylifera's regular intake plays a role in epigenetic modification among children with autism. | ||
| Keywords | ||
| Ajwa dates fruit; Autism; epigenetics modulation; DNA methylation, Expression of DNMT1 and CHD8; Expression modulation of plasma miR-146a and miR-146b; Phoenix dactylifera | ||
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