Phenotypic and Genotypic detection of local MRSA isolates
Kadry, A., Shaker, G., El-Ganiny, A., Youssef, C. (2016). Phenotypic and Genotypic detection of local MRSA isolates. EKB Journal Management System, 25(1), 39-46. doi: 10.21608/zjps.2016.38164
Ashraf Kadry; Ghada Shaker; Amira El-Ganiny; Christiana Youssef. "Phenotypic and Genotypic detection of local MRSA isolates". EKB Journal Management System, 25, 1, 2016, 39-46. doi: 10.21608/zjps.2016.38164
Kadry, A., Shaker, G., El-Ganiny, A., Youssef, C. (2016). 'Phenotypic and Genotypic detection of local MRSA isolates', EKB Journal Management System, 25(1), pp. 39-46. doi: 10.21608/zjps.2016.38164
Kadry, A., Shaker, G., El-Ganiny, A., Youssef, C. Phenotypic and Genotypic detection of local MRSA isolates. EKB Journal Management System, 2016; 25(1): 39-46. doi: 10.21608/zjps.2016.38164

Phenotypic and Genotypic detection of local MRSA isolates

Zagazig Journal of Pharmaceutical Sciences
Article 4, Volume 25, Issue 1, 2016, Page 39-46  XML PDF (462.46 K)
Document Type: Original Article
DOI: 10.21608/zjps.2016.38164
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Authors
Ashraf Kadry email 1; Ghada Shaker2; Amira El-Ganiny2; Christiana Youssef3
1Professor of Microbiology, Faculty of Pharmacy Zagazig University
2Department of Microbiology and Immunology,Faculty of Pharmacy, Zagazig University, Zagazig, 44519, Egypt.
3Department of Microbiology and Immunology,Faculty of Pharmacy, Zagazig University, Zagazig, 44519, Egypt
Abstract
Methicillin-Resistant Staphylococcus aureus (MRSA) infections have become a global health problem particularly in developing countries. MRSA strains are characterized by rapid resistance against different groups of antibiotics.The current study was aimed to identify MRSA isolates phenotypically by disk diffusion method and genoytypically by PCR.
A total of 200 clinical specimens were collected from Zagazig University Hospitals and El- Ahrar Educational Hospital in Zagazig, Egypt, and identified biochemically. Out of these specimens, 117 isolates were identified as MRSA by disk diffusion method (DDM); only 114 of these isolates were identified as MRSA by PCR amplification of mecA gene. The study revealed that PCR was more accurate and rapid than other conventional methods and it is considered the gold standard method for MRSA detection to avoid false positive identification of MRSA.
Keywords
Staphylococcus aureus; MRSA; disk diffusion method (DDM); PCR; mecA
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