Spectrophotometric determination of secnidazole in pure form and pharmaceutical formulation | ||||
| Zagazig Journal of Pharmaceutical Sciences | ||||
| Article 8, Volume 23, Issue 2, 2014, Page 75-87 PDF (1006.35 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/zjps.2014.38187 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Mohamed M. Baraka1; Mohamed E. Elsadek1; Arwa M. Ibrahim2 | ||||
| 1Medicinal Chemistry Department, Faculty of Pharmacy, Zagazig University, P.C.44519, Zagazig, Egypt. | ||||
| 2Pharmacist, Ministry of health, Benghazi, Libya. | ||||
| Abstract | ||||
| Four simple and sensitive spectrophotometric methods were proposed for determination of secnidazole in pure and in tablet forms. The methods (I, II, III) depends on the reduction of secnidazole molecule with zinc dust and hydrochloric acid), forward by coupling reaction between the drug and vanillin reagent in acidic condition (method I), the red colored product was measured atmax 557 nm. Beer’s law was obeyed in the range 5-55 μg/ml. Method II, 1, 2-Naphthoquinone-4- sulphonate sodium react in alkaline media through nucleophilic substitution reaction producing an orange-brown colored product showing maximum absorption at 480 nm. Beer’s law was obeyed in the range 0.2-2.2 μg/ml. Method III, charge transfer complex was formed with tetracyanoethylene with maximum absorbance at 397 nm. Beer’s law was obeyed in the range 0.15-1.2 μg/ml. Method IV based on bromination-oxidation reaction using bromate-bromide mixture with methyl orange as reagent and measuring the absorbance of the unbleached dye at 510 nm. Beer’s law was obeyed in the range 3-8 μg/ml. Under optimized conditions, the methods were applied successfully to the tablets containing secnidazole. The results obtained are in good agreement with those obtained using official and reference methods. | ||||
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