Preparation of 186Re-Cefixime as a Potential Diagnostic and Therapeutic Agent for Bacterial Infection
Talaat, H., Aydia, M., Ibrahim, I., El-Said, H., El-Azony, K. (2019). Preparation of 186Re-Cefixime as a Potential Diagnostic and Therapeutic Agent for Bacterial Infection. EKB Journal Management System, 32(1), 95-103. doi: 10.21608/ejrsa.2019.6992.1059
Hanan M. Talaat; Mohamed I. Aydia; Ismail T. Ibrahim; Hassan El-Said; Khaled M. El-Azony. "Preparation of 186Re-Cefixime as a Potential Diagnostic and Therapeutic Agent for Bacterial Infection". EKB Journal Management System, 32, 1, 2019, 95-103. doi: 10.21608/ejrsa.2019.6992.1059
Talaat, H., Aydia, M., Ibrahim, I., El-Said, H., El-Azony, K. (2019). 'Preparation of 186Re-Cefixime as a Potential Diagnostic and Therapeutic Agent for Bacterial Infection', EKB Journal Management System, 32(1), pp. 95-103. doi: 10.21608/ejrsa.2019.6992.1059
Talaat, H., Aydia, M., Ibrahim, I., El-Said, H., El-Azony, K. Preparation of 186Re-Cefixime as a Potential Diagnostic and Therapeutic Agent for Bacterial Infection. EKB Journal Management System, 2019; 32(1): 95-103. doi: 10.21608/ejrsa.2019.6992.1059

Preparation of 186Re-Cefixime as a Potential Diagnostic and Therapeutic Agent for Bacterial Infection

Egyptian Journal of Radiation Sciences and Applications
Article 9, Volume 32, Issue 1, August 2019, Page 95-103  XML PDF (818.88 K)
Document Type: Original Article
DOI: 10.21608/ejrsa.2019.6992.1059
View on SCiNiTO View on SCiNiTO
Authors
Hanan M. Talaat1; Mohamed I. Aydia2; Ismail T. Ibrahim1; Hassan El-Said2; Khaled M. El-Azony email 2
1Labeled Compounds Department, Hot Labs. Center, Atomic Energy Authority (AEA), Cairo, Egypt
2Radioactive Isotopes and Generators Department, Hot Labs. Center, Atomic Energy Authority (AEA), Cairo, Egypt
Abstract
SEVERAL factors that influence the preparation of 186Re-Cefixime such as the amount of Cefixime and stannous chloride, pH, reaction time and reaction temperature were studied to optimize the labeling conditions to obtain the highest radiochemical yield of 186Re-Cefixime. The radiochemical purity of Rhenium-186 was determined by paper chromatography, while the radiochemical yield and purity of 186Re-Cefixime were determined by electrophoresis and high-performance liquid chromatography (HPLC). The maximum radiochemical yield of 186Re-Cefixime was obtained (96±2.8%) using 2mg Cefixime, 0.3mg carrier added 186Re and 0.5mg stannous chloride at pH 5.5, within 30min at room temperature. The bio-distribution was carried out on three types of mice (normal, sterile infected and bacterial infected). The results show that 186Re-Cefixime is more concentrated in the bacterially infected muscle (septic inflammation) than in the sterile infected muscle (aseptic inflammation). Therefore, 186Re-Cefixime could be used to differentiate between septic and aseptic inflammation.
Keywords
Cefixime; Labeling; Rhenium-186; Biodistribution; Septic and aseptic inflammation
Statistics
Article View: 375
PDF Download: 282