Detection of EnterotoxigenicStaphylococcus aureusin Meat Product Sandwiches Using Multiplex PCR
EL-Maghraby, M., Hassan, M., Hassanin, F., Shawky, N. (2018). Detection of EnterotoxigenicStaphylococcus aureusin Meat Product Sandwiches Using Multiplex PCR. EKB Journal Management System, 35(1), 190-196. doi: 10.21608/bvmj.2018.38362
Marwa Salah EL-Maghraby; Mohamed Ahmed Hassan; Faten Said Hassanin; Nahla Ahmed Shawky. "Detection of EnterotoxigenicStaphylococcus aureusin Meat Product Sandwiches Using Multiplex PCR". EKB Journal Management System, 35, 1, 2018, 190-196. doi: 10.21608/bvmj.2018.38362
EL-Maghraby, M., Hassan, M., Hassanin, F., Shawky, N. (2018). 'Detection of EnterotoxigenicStaphylococcus aureusin Meat Product Sandwiches Using Multiplex PCR', EKB Journal Management System, 35(1), pp. 190-196. doi: 10.21608/bvmj.2018.38362
EL-Maghraby, M., Hassan, M., Hassanin, F., Shawky, N. Detection of EnterotoxigenicStaphylococcus aureusin Meat Product Sandwiches Using Multiplex PCR. EKB Journal Management System, 2018; 35(1): 190-196. doi: 10.21608/bvmj.2018.38362

Detection of EnterotoxigenicStaphylococcus aureusin Meat Product Sandwiches Using Multiplex PCR

Benha Veterinary Medical Journal
Article 20, Volume 35, Issue 1, September 2018, Page 190-196  XML PDF (489.63 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2018.38362
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Authors
Marwa Salah EL-Maghraby1; Mohamed Ahmed Hassan2; Faten Said Hassanin2; Nahla Ahmed Shawky3
1Faculty of Veterinary Medicine, Menofyia University
2Department of Meat Hygiene and Control, Faculty of Veterinary Medicine, Benha University
3Department Food Hygiene,Shebinelkom Lab., Animal Health Research Institute
Abstract
The current work was performed to detect Staphylococcus aureus and their enterotoxin genes as sea, seb, sec andsed among street vended meat products represented by beef burger, kofta and sausage (30 of each) using traditional method of bacteriological isolation and multiplex PCR. The results illustrated that 37 (41.11%) out of 90 meat product samples harbored S.aureuspathogen. Accurately, S.aureus enterotoxin genes were detected in 13 (35.1%) out of 37 isolates. 3 isolate for beef burger samples mostly harboured (2) sea and (1) seb and the other 6 strains was negative for classic enterotoxin genes. Meanwhile, 5 isolates for kofta samples harboured (2) sea, (1) sec, (1)sedand(1) sea&sedgene and the other 7 were negative for classic enterotoxin genes, but 16 isolates of sausage samples harboured (2) sea, (1) seb, (1) mixed seb&sec and (1) mixed sea, sec & sec genes and 11 was negative for classic enterotoxin genes. Furthermore, seais more prevalent in 6 samples (16.2%) followed by sebin 2 samples (5.4%) and sec, sed, sea &sed, seb&secand sea &sec&sed in one sample (2.7%). Finally, Multiplex PCR is efficient in detection of SEs in food. Contamination of meat products with such serous pathogen was discussed.
Keywords
S. aureus; Multiplex PCR
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