Phenotypic and Molecular Characterization of Rubber Degrading Strain Streptomyces Strain E1 | ||||
| Journal of Agricultural Chemistry and Biotechnology | ||||
| Article 4, Volume 8, Issue 4, April 2017, Page 139-142 PDF (221.16 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jacb.2017.38489 | ||||
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| Author | ||||
| E. Ibrahim | ||||
| Faculty of agriculture Department of Genetic South valley university, Qena, Egypt. | ||||
| Abstract | ||||
| Streptomyces sp. strain E1 isolated from soil sample beside the fuel station in Qena governorate, Egypt, this strain able to degraded both synthetic cis-1,4-polyisoprene rubber and natural rubber and to be translucent halos (clear zones) forming on latex overly agar plates. The clear zones formation on natural rubber latex appears after 4-5 days at 30 ºC of incubation. It was also shown that able to utilize some isopronoid compounds as a carbon source and energy. The isolate strain E1 was aerobic, Gram-positive, slightly acidophilic that grows at pH 5.5 – 8.5 and non-motile. Taxonomic characterization of this isolate by 16S rDNA analysis showed highest similarities to the 16S rDNA of Streptomyces setonii (99.8) which is mesophilic actenomycetes. Consequently, bacterial isolate was defined as Streptomyces sp. strain E1. The mineralization experiments were confirmed the capability of rubber degradation for isolate strain. Degradation of isoprenoid compounds and related compounds namely, squalane, phytol, squalene, acetonylacetone, geranylacetone, citronellal and citronellic acid was recognized indicating that the isolate strain has the metabolic capability to degraded some isoprenoid compounds as sources of carbon. | ||||
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