Characterization of Paramyrothecium roridum isolated from soil | ||||
| Journal of Bioscience and Applied Research | ||||
| Volume 10, Issue 4, November 2024, Page 695-712 PDF (2.46 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jbaar.2024.309341.1062 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
janan khorshed  1; Faten N. Mula Abed2; Fahad K. Y. AL-Dulaimi3
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| 1Department of biology | ||||
| 2College of Science, Mosul University, Iraq | ||||
| 3Northern Technical University, College of Agricultural Technology, Plant Production techniques Mosul, Iraq | ||||
| Abstract | ||||
| The fungus Paramyrothecium roridum (Tode) L. Lombard and Crous, previously known as Myrothecium roridum Tode ex Fries, belongs to the family Stachybotryaceae, the species of Myrothecium distributed worldwide in the soil and decaying plant tissues as saprophytic fungi or as pathogens on diverse hosts. The morphology of asexual morph, particularly the characteristics of conidia and conidiophores, was previously used to classify species in this genus, because few features distinguish species within a genus, morphology-based identification alone is inaccurate; consequently, DNA sequence data is useful in identifying species. The fungal isolate was identified according to morphological characteristics. In PDA culture, its olive green conidia were 6-6.5 μm long × 2.3 μm wide, and cylindrical with rounded ends. Conidia formed dark green to black masses on sessile sporodochia in concentric zones, as well as identified based on the molecular approach by using polymerase chain reaction assay of internal transcribed spacer (ITS1 5.8S ITS2) of the rDNA to confirm of Paramyrothecium roredium by using primers (ITS1 and ITS4), the produced band size was 600 bp. The nucleotide sequences of amplification products were analyzed, the results confirmed Paramyrothecium roredium diagnosis. As a results, the isolate Paramyrothecium roridum fungus was recorded as JFF22 isolate in National Center for Biotechnology Information (NCBI) for the first time in Iraq. | ||||
| Keywords | ||||
| Paramyrothecium roridum; media; Polymerase chain reaction; Morphological identification; Scanning electron micrograph | ||||
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