Prevalence, Morphological and Molecular Characterization of Anisakis simplex Larvae in Commercially Important Fishes from Egyptian Markets
Attia, M., Mahdy, O., Soliman, A., Abdelsalam, M., Salem, M. (2024). Prevalence, Morphological and Molecular Characterization of Anisakis simplex Larvae in Commercially Important Fishes from Egyptian Markets. EKB Journal Management System, (), 1-10. doi: 10.21608/ejvs.2024.311109.2305
Marwa Mohamed Attia Attia; Olfat A. Mahdy; Asmaa W Soliman; Mohamed Abdelsalam; Mai A Salem. "Prevalence, Morphological and Molecular Characterization of Anisakis simplex Larvae in Commercially Important Fishes from Egyptian Markets". EKB Journal Management System, , , 2024, 1-10. doi: 10.21608/ejvs.2024.311109.2305
Attia, M., Mahdy, O., Soliman, A., Abdelsalam, M., Salem, M. (2024). 'Prevalence, Morphological and Molecular Characterization of Anisakis simplex Larvae in Commercially Important Fishes from Egyptian Markets', EKB Journal Management System, (), pp. 1-10. doi: 10.21608/ejvs.2024.311109.2305
Attia, M., Mahdy, O., Soliman, A., Abdelsalam, M., Salem, M. Prevalence, Morphological and Molecular Characterization of Anisakis simplex Larvae in Commercially Important Fishes from Egyptian Markets. EKB Journal Management System, 2024; (): 1-10. doi: 10.21608/ejvs.2024.311109.2305

Prevalence, Morphological and Molecular Characterization of Anisakis simplex Larvae in Commercially Important Fishes from Egyptian Markets

Egyptian Journal of Veterinary Sciences
Articles in Press, Corrected Proof, Available Online from 18 November 2024  XML PDF (1.15 MB)
Document Type: Original Article
DOI: 10.21608/ejvs.2024.311109.2305
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Authors
Marwa Mohamed Attia Attia1; Olfat A. Mahdy2; Asmaa W Soliman3; Mohamed Abdelsalam email 4; Mai A Salem5
1Faculty of Veterinary Medicine, Cairo University, Giza
2Department of Parasitology, Faculty of Veterinary Medicine, Cairo University
3National Institute of Oceanography and Fisheries (NIOF), Cairo, Egypt.
4Department of Aquatic Animal Medicine and Management, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt
5Parasitology Department, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt
Abstract
Anisakiasis, caused by nematodes from the Anisakidae family, has become a notable global health issue, primarily due to the increased consumption of raw or undercooked seafood. This study investigated the occurrence and prevalence of Anisakis larvae in commonly sold fish in Egyptian markets. Morphological and molecular techniques were employed to identify the larvae. Our findings revealed a high prevalence of Anisakis larvae, with 85% of Atlantic herring (Clupea harengus) and 52% of Atlantic mackerel (Scomber scombrus) being infected. The mean intensity of infection was 11.17 larvae per infected herring and 4.03 larvae per infected mackerel, while the mean abundance was 9.5 larvae per herring and 2.1 larvae per mackerel. Detailed morphological analysis identified the larvae as Anisakis simplex type I, characterized by specific anatomical features visible under light and scanning electron microscopy. DNA sequencing of the Internal Transcribed Spacer (ITS) region was aligned with GenBank data, confirming the identity of the larvae as A. simplex. This study highlights the significant presence of Anisakis larvae in the Egyptian fish supply, emphasizing the need for public health interventions to mitigate the risks associated with consuming raw or undercooked fish. The application of molecular techniques has enhanced our understanding of the species composition and potential health impacts of Anisakis in Egypt, contributing to global efforts to manage anisakiasis.
Keywords
Anisakis simplex; Atlantic herring; Atlantic mackerel; zoonosis; molecular identification
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