Unraveling the Inflammatory Symphony: Dissecting the Dysregulated NF-κB, IL-6, and IFN-γ in Nephropathy Patients | ||||
| Journal of Bioscience and Applied Research | ||||
| Volume 10, Issue 4, November 2024, Page 744-759 PDF (1.03 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jbaar.2024.392662 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Nazanin Jabbari  1; Parisa Gheibi2; Yousif Badr Makki2; Zahra Azizi3
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| 1Department of Pharmacology, National University of Science and Technology, Dhi-Qar, Iraq | ||||
| 2Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||||
| 3Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran; Iran | ||||
| Abstract | ||||
| Background: Diabetes patients have been known to activate the inflammasome pathway. The current research was carried out to assess the gene expression levels of nuclear factor-κappa B (NF-κB), Interleukin 6 (IL-6), and Interferon Gamma (IFN-γ) in PBMC samples from peripheral blood to find out whether its mRNA could be used as an early marker for renal function deterioration in diabetics. Methods: This empirical study collected serum samples from 60 diabetic patients suffering from nephropathy and 60 healthy controls. Collected blood from the patients was isolated and preserved in the medium at a temperature of -20. Fasting blood sugar (FBS), total cholesterol, triglycerides, HDL-cholesterol, LDL-cholesterol, cholesterol/HDL-LDL, AST, ALT, alkaline phosphatase, and CBC were analyzed. Real-time PCR measured NF-κB, IL-6, and IFN-γ gene expression. Results: Diabetic patients with nephropathy showed increased fasting plasma glucose levels compared to non-diabetic subjects, although there were no significant differences between them regarding cholesterol, triglyceride or other lipid levels, white blood cell count, and red blood cell count. Along with platelet count parameters, the levels of MCV, MCH, MCHC, RDW-CV, AST, ALT, and alkaline phosphatase did not change in the control group. The patient group showed a significant increase in NF-κB and IL-6 gene expression at the level of (P-value <0.001) and also IFN-γ expression at the level of (P-value <0.01) compared to the healthy control group. Conclusion: Due to the elevated level of these genes in nephropathic patients, it can be possibly concluded that NF-κB, IL-6, and IFN-γ are involved in nephropathy complications of diabetes. | ||||
| Keywords | ||||
| Diabetic nephropathy; NF-κB; IL-6; IFN-γ; PBMC | ||||
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