Utility of polymerase chain reaction for molecular detection of bovine herpesvirus1
Elseafy, M., Elkholy, A., Radwan, G. (2014). Utility of polymerase chain reaction for molecular detection of bovine herpesvirus1. EKB Journal Management System, 8(1), 44-55. doi: 10.21608/jcvr.2014.39461
Mai Elseafy; Alaa Elkholy; Gamal Radwan. "Utility of polymerase chain reaction for molecular detection of bovine herpesvirus1". EKB Journal Management System, 8, 1, 2014, 44-55. doi: 10.21608/jcvr.2014.39461
Elseafy, M., Elkholy, A., Radwan, G. (2014). 'Utility of polymerase chain reaction for molecular detection of bovine herpesvirus1', EKB Journal Management System, 8(1), pp. 44-55. doi: 10.21608/jcvr.2014.39461
Elseafy, M., Elkholy, A., Radwan, G. Utility of polymerase chain reaction for molecular detection of bovine herpesvirus1. EKB Journal Management System, 2014; 8(1): 44-55. doi: 10.21608/jcvr.2014.39461

Utility of polymerase chain reaction for molecular detection of bovine herpesvirus1

Journal of Current Veterinary Research
Article 7, Volume 8, Issue 1, 2014, Page 44-55  XML
Document Type: Original Article
DOI: 10.21608/jcvr.2014.39461
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Authors
Mai Elseafy1; Alaa Elkholy1; Gamal Radwan2
1Veterinary Serum and Vaccine Res. Instit., El-Sekka El-Beda St., P.O. Box 131, P.C. 11381 Abbassia, Cairo
2Genetic Engineering and Biotechnology Research Institute, University of Sadat city, Minufiya
Abstract
In the present study, a panel of polymerase chain reactions (PCRs) was compared to conventional diagnostic assays [virus isolation( VI) and indirect immunofluorescence (IFA)] used for detection of bovine herpesvirus-1 (BoHV-1) in nasal swabs collected from calves vaccinated with BoHV-1 gE negative live vaccine (n=18) and from clinically suspected calves (n=159) at different localities of Egypt (El-Sharquia, El-Salhia, El-Qalubeia and El-Behera). PCR assays were carried out utilizing specific primer sets, each targeting one of the viral genes encoding for glycoproteins D (gD), gE and gB (first round and nested gB-based PCR) as well as the thymidine kinase (tk) gene of BoHV-1. Specimens from both control and live BoHV-1 gE negative vaccinated calves were negative for all assays. Whereas, specimens from clinically suspected calves gave variable results as follows: 6/159 (3.7%), 5/159 (3.1%), 15/159 (9.4%), 15/159 (9.4%), 15/159 (9.4%), 18/159 (11.3%), and 16/159 (10.0%) were BoHV-1 positive using VI, IFA, gD-based PCR, tk-based PCR, gE-based PCR, gB- first round PCR and gB- nested PCR, respectively. In conclusion, PCR assays were more sensitive and independent of sample quality than VI or IFA. Moreover, the gE- based PCR was proved reliable for distinction between BoHV-1 gE negative-vaccinated and infected animals. Obtained data emphasized the utility of the BoHV-1 gB nested-PCR as a sensitive, discriminative and rapid diagnostic tool for epidemiological studies and control programs of BoHV-1 infections. Accordingly, it is recommended for routine screening of not only local but also imported animals and biologics for BoHV-1 contamination.
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