Impact of Using Brilliant Cresyl Blue Stain on Oocyte and Embryo Selection: Review Article
Ghoneimy M., K., El-Naby, A., Elraay, M., Youssef, Y., sosa, G., Abou El-Roos, M. (2017). Impact of Using Brilliant Cresyl Blue Stain on Oocyte and Embryo Selection: Review Article. EKB Journal Management System, 48(1), 43-51. doi: 10.21608/ejvs.2017.1244.1016
Karima Ghoneimy M.; Al-shimaa El-Naby; mohamed Elraay; Youssef Youssef; gamal sosa; Mahmoud Abou El-Roos. "Impact of Using Brilliant Cresyl Blue Stain on Oocyte and Embryo Selection: Review Article". EKB Journal Management System, 48, 1, 2017, 43-51. doi: 10.21608/ejvs.2017.1244.1016
Ghoneimy M., K., El-Naby, A., Elraay, M., Youssef, Y., sosa, G., Abou El-Roos, M. (2017). 'Impact of Using Brilliant Cresyl Blue Stain on Oocyte and Embryo Selection: Review Article', EKB Journal Management System, 48(1), pp. 43-51. doi: 10.21608/ejvs.2017.1244.1016
Ghoneimy M., K., El-Naby, A., Elraay, M., Youssef, Y., sosa, G., Abou El-Roos, M. Impact of Using Brilliant Cresyl Blue Stain on Oocyte and Embryo Selection: Review Article. EKB Journal Management System, 2017; 48(1): 43-51. doi: 10.21608/ejvs.2017.1244.1016

Impact of Using Brilliant Cresyl Blue Stain on Oocyte and Embryo Selection: Review Article

Egyptian Journal of Veterinary Sciences
Article 5, Volume 48, Issue 1, June 2017, Page 43-51  XML PDF (643.04 K)
Document Type: Review Artical
DOI: 10.21608/ejvs.2017.1244.1016
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Authors
Karima Ghoneimy M. email orcid 1; Al-shimaa El-Naby2; mohamed Elraay2; Youssef Youssef3; gamal sosa4; Mahmoud Abou El-Roos5
1prof Dr at National Research Center
2Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, Egypt
3National Research Center, Egypt
4Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, Egypt.
5Faculty of Veterinary Medicine, Benha University
Abstract
The success of in vitro embryo production is ultimately dependent on the number and quality of cumulus-oocyte complexes harvested from ovaries. The morphological criteria are routinely used for oocytes selection in most laboratories. There is brilliant cresyl blue stain (BCB) test allowing the selection of developmentally competent oocytes which is a simple, quick, economic and feasible protocol. BCB is known to be a non-invasive methodology that allows the selection of immature oocytes in several species. BCB test determine the intra cellular activity of glucose-6-phosphate dehydrogenase (G6PDH) that gradually decreases its activity as oocytes reach their growth phase. The using of BCB test before IVM is still controversial, so this review describes the use of BCB staining test for oocytes and embryo selection to investigate the impact of application of this stain in in-vitro maturation and embryo production in animals.
Keywords
OOocytes quality; brilliant cresyl blue stain; Embryo Quality; selection; in vitro maturation
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