Molecular Characterization of Proteus mirabilis Recovered From Milk and Dairy Products | ||||
| Egyptian Journal of Veterinary Sciences | ||||
| Articles in Press, Corrected Proof, Available Online from 08 January 2025 PDF (858.02 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejvs.2024.327780.2423 | ||||
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| Authors | ||||
Manal Salah M. A. El-Maghraby1; Ashraf Ashraf awad2; Marwa Salah M. A. El-Maghraby3; Wageh Sobhy Darwish   4; Amany Omar Selim5
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| 1Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Benha University, Benha, Egypt | ||||
| 2Bacteriology, Mycology and Immunology.faculty of veterinary medicine ,Banha Univerisity,Egypt | ||||
| 3Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Menoufia University, Egypt | ||||
| 4Professor of food hygiene, Fac. Vet. Med., Zagazig Univ., Egypt | ||||
| 5Department of Bacteriology, Animal Health Research Institute (AHRI), Benha Branch, Benha, Agriculture Research Center (ARC), Egypt. | ||||
| Abstract | ||||
| Globally, Proteus spp. is widespread in many foods and animals, presenting a significant health obstacle. The present investigation found that Proteus mirabilis (P. mirabilis) was recorded in 19 (12.67%) samples of 150 examined dairy products. The occurrence rates of P. mirabilis in the analyzed samples of raw milk, Kariesh cheese, Tallaga cheese, Feta cheese, yoghurt, and ice cream were 20%, 28%, 16%, 8%, 4%, and 0%, respectively. The antimicrobial resistance of the isolated P. mirabilis strain was assessed, revealing their resistance to several antibiotics. All P. mirabilis isolates exhibited full resistance to erythromycin, whereas 94.7% were resistant to tetracycline and penicillin-G. The detection of the marker gene, the UreR gene, by using PCR confirmed the detection of P. mirabilis. The presence of this gene was observed in all 19 P. mirabilis isolates. The genetic analysis of Proteus spp. was conducted to identify the presence of LuxA, FlaA, mrpA, and hpmA genes, which are known to be associated with virulence in P. mirabilis. Out of the 19 P. mirabilis isolates, the presence of the luxA gene was identified in 12 of them. The presence of the FlaA gene was observed in all P. mirabilis isolates. In conclusion, Kariesh cheese had significantly the highest prevalence rate, followed by raw milk. Therefore, strict hygienic measures should be followed during raw milk collection, and the manufacture of dairy products to avoid contamination of such products with enteric bacteria such as P. mirabilis. | ||||
| Keywords | ||||
| P. mirabilis, dairy products; drug resistance; virulence genes | ||||
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