Influence of Plant Growth Regulators on Micro-Propagation and Somaclonal Variation of Two Strawberry Cultivars. | ||||
| Journal of Agricultural Chemistry and Biotechnology | ||||
| Article 3, Volume 7, Issue 6, June 2016, Page 169-173 PDF (464.84 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jacb.2016.40805 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Rehab Habiba1; Kawther kash1; Noha Sukar2; Eman Abd EL- Moneim2 | ||||
| 1Department of Genetics, Faculty of Agriculture, Mansoura University, Egypt. | ||||
| 2Department of Biological and Environmental Sciences, Faculty of Home Economics, Azhar University, Egypt. | ||||
| Abstract | ||||
| This study was carried out to investigate the effect of some combinations of two plant growth regulators on the multiplication and stability of the two strawberry genotypes, Sweet Charlie (SC.) and Florida (FL.) cultivars. Shoot tips of the two cultivars were cultured on MS medium supplemented with combinations of BA at 3.0 mg/l with three levels of GA3at (0.2, 0.4, 0.6 mg/l). Data were collected after 8 weeks of cultured. The results showed that Sweet Charlie produced better multiplication than the Florida. DNA extracted from leaf tissue of in vitro derived plantletswere analyzed by RAPD-PCR to detect possible drift in genetic stability of micro-propagated plants.The results showed the different fingerprints of RAPD with concentrations of BA and GA3 turn for variation within replicates per concentration. The results showed the highest polymorphism appeared in Florida when was compared to Sweet Charlie, the results indicate that the Sweet Charlie is more stable and would be more suitable for in vitro micro-propagation. | ||||
| Keywords | ||||
| strawberry; Plant growth regulators; micro-propagation; somaclonal variation | ||||
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