Isolation, Characterization and Insecticidal Activity of Methylene Chloride Extract of Cladosporium cladosporioides Secondary Metabolites against Aphis gossypii (Glov.)
Shaker, N., Ahmed, G., El-Sawy, M., Ibrahim, H., Ismail, H. (2019). Isolation, Characterization and Insecticidal Activity of Methylene Chloride Extract of Cladosporium cladosporioides Secondary Metabolites against Aphis gossypii (Glov.). EKB Journal Management System, 10(2), 115-119. doi: 10.21608/jppp.2019.40896
N. O. Shaker; G. M. M. Ahmed; M. M. El-Sawy; H. Y. E. Ibrahim; H. N. A. Ismail. "Isolation, Characterization and Insecticidal Activity of Methylene Chloride Extract of Cladosporium cladosporioides Secondary Metabolites against Aphis gossypii (Glov.)". EKB Journal Management System, 10, 2, 2019, 115-119. doi: 10.21608/jppp.2019.40896
Shaker, N., Ahmed, G., El-Sawy, M., Ibrahim, H., Ismail, H. (2019). 'Isolation, Characterization and Insecticidal Activity of Methylene Chloride Extract of Cladosporium cladosporioides Secondary Metabolites against Aphis gossypii (Glov.)', EKB Journal Management System, 10(2), pp. 115-119. doi: 10.21608/jppp.2019.40896
Shaker, N., Ahmed, G., El-Sawy, M., Ibrahim, H., Ismail, H. Isolation, Characterization and Insecticidal Activity of Methylene Chloride Extract of Cladosporium cladosporioides Secondary Metabolites against Aphis gossypii (Glov.). EKB Journal Management System, 2019; 10(2): 115-119. doi: 10.21608/jppp.2019.40896

Isolation, Characterization and Insecticidal Activity of Methylene Chloride Extract of Cladosporium cladosporioides Secondary Metabolites against Aphis gossypii (Glov.)

Journal of Plant Protection and Pathology
Article 4, Volume 10, Issue 2, February 2019, Page 115-119  XML PDF (498.51 K)
Document Type: Original Article
DOI: 10.21608/jppp.2019.40896
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Authors
N. O. Shaker1; G. M. M. Ahmed2; M. M. El-Sawy1; H. Y. E. Ibrahim2; H. N. A. Ismail2
1Applied Organic Chemistry, Faculty of Science (Girls), Al-Azhar University
2Plant Protection Research Institute, ARC, Dokki, Giza, Egypt.
Abstract
 
The cotton aphid, Aphis gossypii Glover is a serious plant pests causing serious damage to the cultivated crops. Entomopathogenic fungi were early used for controlling aphids. Cladosporium cladosporioides is one of the promising candidates fungus can be used as a microorganism or can be used as a source of toxins against insect pests. Present research studied the chemical constituents of methylene chloride extracts of C.  cladosporioides. It revealed 17 volatile compounds when analyzed by GC/MS technique. Also, two major compounds were isolated and identified as Pentadecanoic acid methyl ester (Compound 1) and Lupeol (Compound 2). Furthermore, the insecticidal activity of methylene chloride extracts of C.  cladosporioides was tested against both adults and nymphs of cotton aphid, A. gossypii. It showed the most effective against nymphs of  LC50 of 212.2319 ppm, LC90 of 1407.5663 ppm and toxicity index of 100%, while, it showed LC50 of 276.75 ppm, LC90 of 1902.15 ppm and toxicity index of 76.69% against adult stage.
Keywords
Cladosporium cladosporioide; secondary metabolites; methylene chloride extracts; insecticidal activity and Aphis gossypii
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