EFFECT OF DROUGHTAND COMBINED SALT AND HEAT STRESSES ON GERMINATION AND SEEDLING GROWTH PATTERNS OF LENTIL | ||||
| Zagazig Journal of Agricultural Research | ||||
| Article 1, Volume 46, Issue 3, May and June 2019, Page 595-607 PDF (377.43 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/zjar.2019.40944 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Ahmed H.I. Hamdi  1; Rasha Y.S. Abd El-Khalek2; Hania A.M. Eraky2
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| 1Food Legumes Res. Section, Field Crops Res. Inst. (FCRI), Agric. Res. Cent. (ARC), Giza Egypt | ||||
| 2Seed Technol. Res. Section, FCRI, ARC, Giza Egypt | ||||
| Abstract | ||||
| The present study aimed to investigate the effect of combined salt (three salinity levels of 0.0M, 0.1M and 0.2M NaCl), heat (20 and 30oC) and drought (without drought and drought stress by polyethylene glycol (PEG) at 8000 ppm) stresses on seed germination, seed and seedlings vigor traits of three lentil cultivars (Giza 9, Giza 51 and Sinai 1). Increasing temperature from 20oC to 30oC significantly decreased most studied characters. Both salinity levels significantly decreased all traits comparing with the control treatment (0 salt NaCl). All traits were more sensitive to 0.2M.than 0.1M.NaCl. Effects of drought were insignificant on shoot length, root length, seedling vigor index and root/shoot ratio. Drought significantly decreased seed germination by 5.6% and seed vigor by 4.87%. Cultivar Giza 9 was more tolerant to drought than the other two cultivars. Gel electrophoresis (SDS-PAGE) analysis for lentil cultivars under salt stress and drought stress at two different temperatures produced new protein heat shock protein (HSP) presented under 30oC and was absent under control treatment. The applied laboratory technique is valid to be used for screening large number of lentil accessions for their performance under studied stresses as a rapid technique before screening them for such stresses under field condition. | ||||
| Keywords | ||||
| Lentil (Lens culinaris subsp. Culinaris Medikus); salinity stress; Drought stress; Germination; Seedling characters; SDS protein electrophoresis; lentil | ||||
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