SOME FACTORS AFFECTING MICROPROPAGATION OF Haplophyllum tuberculatum L | ||||
| Zagazig Journal of Agricultural Research | ||||
| Article 4, Volume 46, Issue 3, May and June 2019, Page 635-647 PDF (1.08 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/zjar.2019.40947 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Sonia A.S. Abdallah*  ; M. A.M. Ali; M. A. El-Mekawy; M. M. Abd El-Hameed
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| Plant Prod. Dept., Fac. Environ. Agric. Sci., Arish Univ., Egypt | ||||
| Abstract | ||||
| This study was carried out at Plant Tissue Culture Laboratory, Faculty of Environmental Agricultural Sciences, El-Arish University, North Sinai Governorate, Egypt during 2014 to 2017. Effect of three medium types, Murashige and Skoog (MS), Linsmaier and Skoog (LS) and Lloyd and McCown (WPM), two explant types (shoot-tip and single node cutting), three cytokinin types (Benzyladenine, Kinetin and 2-isopenteny1 adenine), two additives; viz., s (thiamine and pyridoxine hydrochloride) and amino acids (asparagine and arginine) and two gelling agents (guar seed powder and locust bean seed powder) combined with agar on micropropagation of Haplophyllum tuberculatum were investigated. For rooting, the effect of three auxins (Indole acetic acid, Indol butyric acid and Naphthalene acetic acid) were studied in combination with three medium strengths (full, half and quarter) combined with the best mixture (0.50 mgl-1 Kin + 6 gl-1 agar with 2 gl-1guar seed powder) of gelling agents. Obtained results showed that Murashige and Skoog (MS) was the best medium with single node explant as starting material during establishment stage. Multiple shoots were obtained on MS medium supplemented with 1.0 mgl-1 Kinetin (Kin) combined with 0.1 mgl-1 α-naphthalene acetic acid (NAA). Thiamine and arginine were the best vitamins and amino acid at 0.1 mgl-1 and 0.50 mgl-1, respectively. Agar at 6 gl-1 + guar seed powder at 2 gl-1 was the best gelling agent treatment. Half strength MS medium with 0.50 mgl-1 Kin + 0.5 mgl-1 NAA and 6 gml-1 agar with 2 gml-1 guar seed powder was the best combination for rooting were successfully (85% survival) acclimatized in mixture of peatmoss, vermiculite, washed sterilized sand and perlite (1:1:1:1, V/V/V/V). | ||||
| Keywords | ||||
| Haplophyllum tuberculatum; micropropagation; Plantlets; vitamins; kinetin; auixun; multiplication; Rooting; Acclimatization | ||||
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