Production of Thermostable Protease From"Bacillus amyloliquefaciens":An Egyptian Marine Isolate | ||||
| Journal of Agricultural Chemistry and Biotechnology | ||||
| Article 1, Volume 7, Issue 8, August 2016, Page 227-234 PDF (813.73 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jacb.2016.41064 | ||||
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| Authors | ||||
| Hassnaa EL-Eskafy; Marwa Abdel-Hamid; R. Abbas; H. Hamza; A. El- Zanaty | ||||
| Microbial Biotechnology Dept., Genetic Engineering and Biotechnology Institute, Sadat City University, Egypt | ||||
| Abstract | ||||
| Proteases represent an essential group of enzymes that are widely produced and used industrially, thus the need for new microbial isolates with new features is of utmost necessity for industrial applications. A locally marine bacterium, isolated from Red Sea region in Egypt was able to produce thermostable proteases, the isolate was identified morphologically, biochemically, and confirmed molecularly by 16S rRNA sequencing with 98% similarity toBacillus amyloliquefaciens. It exhibited optimum activity of 363.4 U/mLat 65°C and pH 7 for ten min .Both ammonium sulphate and Sephadex G-100 purification methods reduced the specific activity to 256.4 and 153.4 U/mL. However, the activity increased 3.8 folds when Tween-80 was used as surfactant. Genetic background of the protease genes in Bacillus amyloliquefacienswas analyzed using bioinformatics database for the proteases amino acids sequences in the desired bacteria; and it specified that Bacillus amyloliquefacienshas five different protease genes; these genes encode for various peptidase family groups. The variation in the peptidase family provides the protease enzymes with many features making them able to remain active under various environmental stresses. The overall results showed promising thermostable proteases isolated from local marine Egyptian bacterium; that can be used potentially in many industrial applications. The produced Enzyme showed good activity between 65°C and 85°C. While the addition of metal ions inhibited the enzyme activity. | ||||
| Keywords | ||||
| Thermophilic; Protease; Marine bacteria; Bacillus amyloliquefaciens; Peptidases family | ||||
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