Can L-carnitine prevent Cadmium-Induced Damage of the Developing Hippocampus? | ||||
| Egyptian Journal of Histology | ||||
| Article 2, Volume 43, Issue 1, March 2020, Page 16-35 PDF (24.74 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2019.13391.1124 | ||||
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| Authors | ||||
AYMAN S. AMER  ; Faten Y. Mahmoud; Ashraf H. Abd El-Hakem; Mariam W. Fedal
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| Department of Human Anatomy and Embryology, Faculty of Medicine, Assiut University, Egypt | ||||
| Abstract | ||||
| Background: Cadmium is used in everyday life aspects. Hippocampus plays important role in the memory process and could be affected by cadmium exposure. L-carnitine is antioxidant, its role in neuroprotection is still not known. Aim of Work: To assess effects of pre- and postnatal cadmium exposures on structure of principle cells (pyramidal and granular) of hippocampus in albino rats, and the potential role of L-carnitine. Material and Methods: Pregnant female albino rats were used and divided into two major groups: I- Pregnancy and IILactation, each was further equally subdivided into 4 subgroups: Group Ia- Control. Group Ib- received cadmium from pregnancy day (PD)10 until delivery. Group Ic- received L-carnitine from PD7 until delivery. Group Id- received both L-carnitine from PD7 + cadmium from PD10 until delivery. Group IIa- Control. Group IIb- received cadmium from day 0-21. Group IIc- received L-carnitine from day 0-21. Group IId- received both L-carnitine+cadmium from day 0-21. Cadmium chloride was given orally by gastric lavage 4 mg/kg bodyweight/day. L-carnitine dose was given as 200 mg/kg/day orally by gastric lavage. The hippocampal specimens were collected from twelve male offspring of both age subgroups (newborn and 21 day old; 96 rats total) and were processed for light, electron microscopy and morphometry. Results: In cadmium received rats principle cells showed degenerative changes as pyknosis, cytoplasmic vacuoles, damaged mitochondria, indentation of nuclear membrane, and the morphometric data showed highly significant reduction in principle cells number and decreased principle cell layer thickness as compared to control. In cadmium+L-carnitine received rats the principle cells showed a very few swollen cells with little vacuoles, mildly dilated rough endoplasmic reticulum, and the morphometric results were improved approaching those of the control. Conclusion: cadmium damaged principle cells of dentate gyrus, CA3 and CA1 fields of the rat hippocampus. L-carnitine reduced the cadmium-induced neuronal damage | ||||
| Keywords | ||||
| Cadmium; hippocampus; L-carnitine; postnatal study; ultrastructure | ||||
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