Induction of Antimicrobial Peptides in the Hemolymph of Spodoptera littoralis Larvae Following Treatment with Salmonella typhimurium
Radwan, M., Mo'men, S., Abdou, M., El-Gohary, E., ElShafe, A., Barakat, E. (2019). Induction of Antimicrobial Peptides in the Hemolymph of Spodoptera littoralis Larvae Following Treatment with Salmonella typhimurium. EKB Journal Management System, 12(4), 9-21. doi: 10.21608/eajbsa.2019.41374
Marwa H. Radwan; Shaimaa A. A. Mo'men; Mohamed A. Abdou; El-Gohary E. El-Gohary; Akila M. ElShafe; Emad M.S. Barakat. "Induction of Antimicrobial Peptides in the Hemolymph of Spodoptera littoralis Larvae Following Treatment with Salmonella typhimurium". EKB Journal Management System, 12, 4, 2019, 9-21. doi: 10.21608/eajbsa.2019.41374
Radwan, M., Mo'men, S., Abdou, M., El-Gohary, E., ElShafe, A., Barakat, E. (2019). 'Induction of Antimicrobial Peptides in the Hemolymph of Spodoptera littoralis Larvae Following Treatment with Salmonella typhimurium', EKB Journal Management System, 12(4), pp. 9-21. doi: 10.21608/eajbsa.2019.41374
Radwan, M., Mo'men, S., Abdou, M., El-Gohary, E., ElShafe, A., Barakat, E. Induction of Antimicrobial Peptides in the Hemolymph of Spodoptera littoralis Larvae Following Treatment with Salmonella typhimurium. EKB Journal Management System, 2019; 12(4): 9-21. doi: 10.21608/eajbsa.2019.41374

Induction of Antimicrobial Peptides in the Hemolymph of Spodoptera littoralis Larvae Following Treatment with Salmonella typhimurium

Egyptian Academic Journal of Biological Sciences. A, Entomology
Article 2, Volume 12, Issue 4, August 2019, Page 9-21  XML PDF (1.24 MB)
Document Type: Original Article
DOI: 10.21608/eajbsa.2019.41374
View on SCiNiTO View on SCiNiTO
Authors
Marwa H. Radwan; Shaimaa A. A. Mo'men; Mohamed A. Abdou; El-Gohary E. El-Gohary; Akila M. ElShafe; Emad M.S. Barakat
Department of Entomology, Faculty of Science, Ain Shams University, Abbassia, Cairo, Egypt.
Abstract
   This study investigated the efficiency of intrahaemocoelic injection of sublethal concentration (LC20 = 5.7×103 cells/ml) of Salmonella typhimurium in the induction of immune response in 5th larval instar of Spodoptera littoralis. The role of antimicrobial peptides (AMPs) induced in the larval hemolymph was examined as natural antibiotics against different Gram-negative (G-ve) or Gram-positive (G+ve) bacteria. Where the larval plasma injected with Salmonella showed a significant antimicrobial activity against different strains of pathogenic bacteria (Staphylococcus aureus, Salmonella typhimurium, Escherichia coli and Enterococcus faecalis). The biochemical characterization of the immune hemolymph indicated drastic changes in both the total protein content and the protein banding patterns following bacterial injection. The total hemolymph proteins (THPs)decreased significantly 48 h post-Salmonella-injection. The hemolymph proteins profile was also qualitatively changed through the induction of new proteins and the disappearance of others simultaneously, which may be attributed to their participation in the immune reactions. The appearance of six new synthesized protein bands that were found to be specific for Salmonella injection, with a characteristic band of MW ~22 KDa predicting that this protein band may be Attacin-like AMP. 
Keywords
Spodoptera littoralis; Salmonella typhimurium; LC50; Immunization; cell-free hemolymph; Electrophoresis; antimicrobial peptides; Gram-negative and Gram-positive bacteria
Statistics
Article View: 313
PDF Download: 433