Toxicological and Biochemical Effects of Lufenuron and Rice Bran on Desert Locust, Schistocerca gregaria (Forskal) (Orthoptera: Acrididae) | ||||
| Egyptian Academic Journal of Biological Sciences, F. Toxicology & Pest Control | ||||
| Article 5, Volume 11, Issue 2, June 2019, Page 37-58 PDF (1.31 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/eajbsf.2019.41514 | ||||
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| Authors | ||||
| Noura M. Mahdy1; Mona I. Mohammed2; Mohamed A. Abdou2; Shimaa S. Ahmed2; Naji S. Badawy1 | ||||
| 1Plant Protection Research Institute, Agricultural Research Center, Giza, Egypt | ||||
| 2Department of Entomology, Faculty of Science, Ain Shams University, Cairo, Egypt | ||||
| Abstract | ||||
| Lufenuron (chitin synthesis inhibitors) and Oryza sativa bran extract (waste product) exhibited insecticidal activity on females of Schistocerca gregaria when they were treated as one day old of the 5th nymphal instar with different concentrations of tested compounds. Some biological aspects, failure of ecdysis to adults, adult mortality, prolongation in the age of last nymphs and developmental events were investigated after treatment. Quantitative and qualitative determination of protein and DNA contents were investigated in normal and affected eggs which resulted from females Schistocerca gregaria treated as one day old 5th nymphal instarwith LC50 ofLufenuron and Oryza sativa bran extract during embryogenesis. The total protein content of normal eggs at 0-day old, was 81.07 mg protein/gm eggs, after one day of oviposition the total protein increase significantly (p<0.05). Then the protein content, during the early incubation period, to the 4th day post oviposition (pop) significantly (p<0.05) declined sharply to 38.74mg protein / gm eggs. While in the 7th, 11th &12th it was decreased significantly (p<0.05) during the late organogenesis stage. The treatment resulted a significant decrease in the total protein of rice bran extract affected eggs compared with the control at (1, 11& 12) days old eggs, and a significant increase at 0 and 4 days pop compared with control. Treatment with Lufenuron resulted a significant decrease of total protein in (0, 1 & 12) days old eggs compared with the control but there were no significant increase at 4, 7 and 11 old eggs. A total of (10 -12) protein bands were separated by electrophoresis during normal embryogenesis of Schistocerca gregaria. Fraction protein of affectedeggs with LC50 of Lufenuron and rice bran extract resulted a separation of (10 – 14) and (11- 14) protein bands with molecular weights from 12 to 325 KDa. Data recorded significant differences between DNA content of normal and the affected Schistocerca gregaria eggs throughout embryogenesis (0-12 days) post-oviposition | ||||
| Keywords | ||||
| Schistocerca gregaria; lufenuron; rice bran; protein; DNA | ||||
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