Polymorphism of Toll-like receptors type 2 gene in Neonatal sepsis in NICU of Zagazig University Children Hospital
Hassan, A., Attia, T., Mokhtar, W., Fakhr, A. (2020). Polymorphism of Toll-like receptors type 2 gene in Neonatal sepsis in NICU of Zagazig University Children Hospital. EKB Journal Management System, 26(3), 405-413. doi: 10.21608/zumj.2019.14651.1324
Ahmed Hassan; Tarek Hamed Attia; Wessam Mokhtar; Ahmed Elsadek Fakhr. "Polymorphism of Toll-like receptors type 2 gene in Neonatal sepsis in NICU of Zagazig University Children Hospital". EKB Journal Management System, 26, 3, 2020, 405-413. doi: 10.21608/zumj.2019.14651.1324
Hassan, A., Attia, T., Mokhtar, W., Fakhr, A. (2020). 'Polymorphism of Toll-like receptors type 2 gene in Neonatal sepsis in NICU of Zagazig University Children Hospital', EKB Journal Management System, 26(3), pp. 405-413. doi: 10.21608/zumj.2019.14651.1324
Hassan, A., Attia, T., Mokhtar, W., Fakhr, A. Polymorphism of Toll-like receptors type 2 gene in Neonatal sepsis in NICU of Zagazig University Children Hospital. EKB Journal Management System, 2020; 26(3): 405-413. doi: 10.21608/zumj.2019.14651.1324

Polymorphism of Toll-like receptors type 2 gene in Neonatal sepsis in NICU of Zagazig University Children Hospital

Zagazig University Medical Journal
Article 6, Volume 26, Issue 3, May 2020, Page 405-413  XML PDF (680.56 K)
Document Type: Original Article
DOI: 10.21608/zumj.2019.14651.1324
View on SCiNiTO View on SCiNiTO
Authors
Ahmed Hassan email 1; Tarek Hamed Attia2; Wessam Mokhtar3; Ahmed Elsadek Fakhrorcid 4
1Pediatric department, Faculty of medicine, Zagazig University, Zagazig, Egypt
2Pediatrics department, faculty of medicine, Zagazig University, Zagazig, Egypt.
3professor of pediatrics, faculty of medicine, zagazig university
4Microbiology and Immunology Department
Abstract
Introduction: Neonatal sepsis is a major cause of morbidity and mortality among newborn infants. Sepsis is a complex syndrome that is initiated by infection and is characterized by a systemic inflammatory response. The inflammatory cascade is a complicated process that involves humoral and cellular responses, complement, and cytokine cascades. Recently, genetic variation in crucial genes in the inflammatory response and coagulation pathways has been the focus for innovative research aiming to elucidate the mechanisms behind sepsis, in particular, identification of genetic variation in TLRs.
Objectives: to investigate the association between the prescence of certain genetic polymorphisms of toll-like receptor type 2 genotype and risk, severity, prognosis and outcome of gram-positive bacterial sepsis in neonates in NICU at Zagazig University Childern Hospital.
Methods: This is a case–control study , 72 subjects were included in the present study divided into two groups; group I : 36 healthy newborns as control group, group II: 36 bacteremic cases with early or late onset neonatal sepsis. For Detection of TLR2 (Arg753Gln) polymorphisms, blood specimens were collected in vacutainer tubes containing EDTA then DNA extraction was performed and determination of the TLR 2 gene polymorphisms was accomplished by PCR followed by RFLP.
Results: TLR2 (Arg753Gln) polymorphism was detected in 3 subjects (8.3%) in the sepsis group, while it was not detected in the control group
Conclusion: There is no significant association between TLR2 Arg753Glu polymorphism and severity or prognosis of gram-positive bacterial sepsis in neonates.
Keywords
Sepsis; Toll-like receptor 2; Single nucleotide polymorphism; Genetic susceptibility; Newborn infant
Statistics
Article View: 293
PDF Download: 340