An Easy and Simple Approach for Biosynthesis of Selenium Nanoparticles; Characterization, Radiolabeling and Biomedical Application

Elkouly, Heba I.; Nasr, Zainab S.; Hassane, Mohamed K.

doi:10.21608/ejchem.2025.338679.10892

	An Easy and Simple Approach for Biosynthesis of Selenium Nanoparticles; Characterization, Radiolabeling and Biomedical Application
Egyptian Journal of Chemistry
Volume 68, Issue 11, November 2025, Page 543-555 PDF (402.67 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2025.338679.10892
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Authors
Heba I. Elkouly ¹; Zainab S. Nasr ²; Mohamed K. Hassane ²
¹Botany & Microbiology Department, Faculty of Science, Al-Azhar University (Girls Branch), Cairo, Egypt, P.O.11754.
²Labeled Compounds Department., Hot Labs Center, Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt.
Abstract
Nanomaterials biogenic approaches of synthesis exceed chemical ways in simplicity, toxicity and generation of homogeneous colloidals. Herein, a rare approach for biosynthesis of SeNPs is described where a rapid myogenic method was applied. The endophytic fungus isolate, Asperigillus terrus H1 with accession no. PQ550684, that isolated for the first time from Rumex thyrsiflorus was used for the bio-reduction of sodium selenite contained in the growth medium to produce selenium nanoparticles. Change in color of the culture medium to orange-red signifies the formation of nano-selenium. DLS, TEM and ICP-OES techniques demonstrated the formation of monodispersive amorphous selenium nanoparticles with average hydrodynamic size of 55.76 nm with -25.6 particles charge. The synthesized SeNPs were checked for their in-vitro cytotoxicity against normal human lung fibroblast cells (MRC-5) and human lung adenocarcinoma cell line (A549) which giving IC50 of 485.16±13.71 and 196.25± 4.23 µg/ml respectively. Their ability to be applied in biomedical applications was studied in-vivo through labeling them with 99mTc and tracing their distribution in normal and tumor-bearing mice. The produced mycogenic SeNPs showed excellent physical and biological characteristics that could make them a promising nanoplatform for tumor molecular imaging.
Keywords
Biogenic nanoparticles; Selenium; endophyte Asperigillus terrus H1; Tumor Imaging; Radiolabeling


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