EFFECT OF PLANT GROWTH REGULATORS ON CALLUS INDUCTION FROM SEEDLING EXPLANTS OF Ammi visnaga L. AND PHENOLIC COMPOUNDS CONTENT | ||||
| Journal of Productivity and Development | ||||
| Article 3, Volume 21, Issue 1, January 2016, Page 55-66 PDF (714.67 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpd.2016.42258 | ||||
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| Authors | ||||
| Sayed Hussein; Heba Shahin; Yasseen Yasseen | ||||
| Genetic Engineering and Biotechnology Research Institute (GEBRI), Sadat City University, Sadat City, P.O. Box 79/22857, Egypt | ||||
| Abstract | ||||
| Ammi visnaga (L.) Lam. Belong to family Umbelliferae. During the present study an efficient in vitro protocol has been standardized viz, callus production from seedling explants. Best callus production from seedling explants was obtained on MS medium supplemented with 0.5mg/l NAA +0.05/1BA. Callus was also obtained when MS medium was fortified with 1mg/1NAA+0.5mg/1BA, 1mg/1 (2, 4D+0.5 mg/1BA and 3mg/l PCIB. But the percent culture response on these concentrations was lesser. The lowest amount of callus was found to be on MS medium containing 1mg/l PCIB 9(1) callus amount. Callus of explants were grown on half MS media gave the significant highest value (3641) of phenolic content, followed by callus of free-growth regulators MS medium (3509.5). While the lowest value (7) was observed with callus obtained from seedling cultured on MS medium contained 1 mg/1 2, 4-D+ 0.5 mg/1K. Conclusively, the Ammi visnaga callus formation can be obtained from different concentrations of growth regulators. The growth regulators also had a significant impact on the amount of callus produced.The efficiency of callus formation depended on the hormone concentrations and the proportion between NAA and BA. The best results were achieved on the medium containing 1mg/lNAA and 0.05mg/lBA. The callus of Half MS plant gave the significant highest value of phenolic content, followed by callus of Free-growth regulators MS medium. | ||||
| Keywords | ||||
| Ammi visnaga (L.); NAA; PCIB; callus formation; 2; 4-D and phnolic compounds | ||||
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