PHYTOHORMONES SCREENING OF Drosera capensis AS INSECTIVOROUS PLANTS AND ITS EXTRACT EFFECT ON in-vitro GROWTH AND DEVELOPMENT OF DATE PALM (Phoenix dactylifera cv. Bartamouda) | ||||
| Journal of Productivity and Development | ||||
| Article 6, Volume 15, Issue 2, April 2010, Page 223-235 PDF (348.85 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpd.2010.42413 | ||||
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| Authors | ||||
| Ibrahim Ibrahim* 1; Hamdy Emara1; Abdel-Moneam El-Banna2; Ibrahim Shams El-Din2 | ||||
| 1Plant Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), Minufiya University. | ||||
| 2Central Laboratory of Date Palm Research and Development, Agricultural Research Center, Giza, Egypt. | ||||
| Abstract | ||||
| Carnivorous or insectivorous plants belong to several botanical families, the most important of them is Droseraceae, which includes Drosera plants. Some economic substances are extracted from Drosera. Tissue culture technique provides the best way to obtain as high and clean quantity as possible of the biomass needed to obtain these substances. This study aimed to evaluate the Drosera capensis content from phytohormones. Also studying the effect of Drosera capensis leaf and root extracts as plant growth substances on in-vitro growth of one of the most important crops that is Phoenix dactylifera cv. Bartamouda. The amounts of phytohormones in this plant (mg 100g-1 fresh weight) were as following: Indole acetic acid in leaf was 2.055, while in case of root was 2.291. Zeatine in leaf was 1.609, while in case of root was 0.418. Other cytokinins in leaf was 18.791, while in case of root was 1.003. Gibberellic acid in leaf was 70.938, while in case of root was 86.59. Abscissic acid in leaf was 0.500, while in case of root was 0.158. Concentrations of the extract of Drosera capensis leaves and roots were applied at different ratios in in-vitro experiments of date palm cv. Bartamouda. The results revealed that Drosera capensis root extract had a significant effect on fresh weight of date palm embryogeneic callus as the best result (4.63 g) was observed with using Drosera capensis root extract at 3.0 ml L-1. Using of Drosera capensis root extract at 0.05 ml L-1 gave rise to higher number of mature embryos. The highest significant shoot number (21 shoots) of date palm was obtained with using 1.0 ml L-1 Drosera capensis leaf extract. Also the length of date palm shoots increased significantly by using the same concentration of Drosera capensis leaf extract and reached 3.3cm. Finally, in-vitro date palm cultivation can be achieved with MS medium supplemented with Drosera capensis extract as a source of phytohormones at different micropropagation stages. | ||||
| Keywords | ||||
| Drosera capensis extract; Phytohormones; In-vitro; date palm cv. Bartamouda; Embryogeneic calli; Mature embryos; Shoot formation | ||||
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