MOLECULAR CHARACTERIZATION AND HETEROSIS OF SOME INBRED LINES OF MELON (Cucumis melo) | ||||
| Egyptian Journal of Plant Breeding | ||||
| Volume 21, Issue 2, April 2017, Page 227-244 PDF (907.42 K) | ||||
| Document Type: Original research | ||||
| DOI: 10.12816/ejpb.2017.424334 | ||||
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| Abstract | ||||
| Seven inbred lines of Cucumis melo L. from Egypt were molecularly characterized using ten primers for RAPD-PCR. Genetic relationship was studied by obtaining dendrogram from the combined molecular data. Dendrogram results revealed two main clusters, one cluster containing Koz El-Assal (P1) and the other cluster contains the rest of the parents, with similarity coefficient of 0.09. Only the first 5 parents were used according to their phylogeny tree and genetic distance. Those parents were cultivated at El-Sabahia Horticulture Research Station, Alexandria Governorate. Fourteen horticulture traits were measured. Analysis of variance data revealed highly significant differences among the studied genotypes for all characters, except for number of branches/plant, which indicated difference in the genetic potential of the populations with respect to these characters. Mean squares indicated fluctuations in the environmental conditions from a year to another concerning plant length and fruit maturity date. P1xP2 had high mean performance values for plant length, number of branches/plant, maturity date and placenta hardness, while P2xP4 had high values for fruit number/plant, total fruit yield/plant, placenta hardness and fruit netting degree. Heterosis showed significant values for good characters in three crosses: (P1xP2), (P2xP4) and (P1xP5). Positive and significant correlation coefficients were exhibited among the following characters: plant length with each of number of branches/plant and fruit shape index, number of branches/plant with fruit shape index (0.44*), flowering date with each of average fruit number/plant (0.51*) and placenta hardness (0.59**), maturity date with placenta hardness and netting degree, average fruit weight and total yield/plant, netting degree, T.S.S and moisture content, and finally total yield with netting degree (0.69**). | ||||
| Keywords | ||||
| Cucumis melo; Melon; RAPD-PCR; Morphological characters; Heterosis | ||||
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