SOMATIC EMBRYOGENESIS AND PLANT REGENERATION INDUCED FROM MATURE SEEDS, COTYLEDONS AND SHOOT TIPS OF CUCUMBER.
Khidr, Y., El-Absawy, E., Mahmoud, A., Hasan, M., Hemeida, A. (2012). SOMATIC EMBRYOGENESIS AND PLANT REGENERATION INDUCED FROM MATURE SEEDS, COTYLEDONS AND SHOOT TIPS OF CUCUMBER.. EKB Journal Management System, 17(2), 193-209. doi: 10.21608/jpd.2012.42464
Yehia Khidr; El-Sayed El-Absawy; Amal Mahmoud; Mohamed Hasan; Alaa Hemeida. "SOMATIC EMBRYOGENESIS AND PLANT REGENERATION INDUCED FROM MATURE SEEDS, COTYLEDONS AND SHOOT TIPS OF CUCUMBER.". EKB Journal Management System, 17, 2, 2012, 193-209. doi: 10.21608/jpd.2012.42464
Khidr, Y., El-Absawy, E., Mahmoud, A., Hasan, M., Hemeida, A. (2012). 'SOMATIC EMBRYOGENESIS AND PLANT REGENERATION INDUCED FROM MATURE SEEDS, COTYLEDONS AND SHOOT TIPS OF CUCUMBER.', EKB Journal Management System, 17(2), pp. 193-209. doi: 10.21608/jpd.2012.42464
Khidr, Y., El-Absawy, E., Mahmoud, A., Hasan, M., Hemeida, A. SOMATIC EMBRYOGENESIS AND PLANT REGENERATION INDUCED FROM MATURE SEEDS, COTYLEDONS AND SHOOT TIPS OF CUCUMBER.. EKB Journal Management System, 2012; 17(2): 193-209. doi: 10.21608/jpd.2012.42464

SOMATIC EMBRYOGENESIS AND PLANT REGENERATION INDUCED FROM MATURE SEEDS, COTYLEDONS AND SHOOT TIPS OF CUCUMBER.

Journal of Productivity and Development
Article 3, Volume 17, Issue 2, April 2012, Page 193-209  XML PDF (436.65 K)
Document Type: Original Article
DOI: 10.21608/jpd.2012.42464
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Authors
Yehia Khidr email 1; El-Sayed El-Absawy2; Amal Mahmoud1; Mohamed Hasan2; Alaa Hemeida2
1Department of Plant Biotechnology, Genetic Engineering and Biotechnology Research Institute, Sadat City, Menofia University
2Department of Bioinformatics, Genetic Engineering and Biotechnology Research Institute, Sadat City, Menofia University
Abstract
Commercial cucumber cultivars were explored for embryogenesis and plant regeneration induced in somatic tissues on plant growth regulators.  An efficient in vitro regeneration protocol for Cucumis sativus L via somatic embryogenesis has been developed. Embryogenic callus  cultures  were  established  from  mature seeds, cotyledons and shoot tips  on Murashige and Skoog (MS) medium  containing  87.64  µM sucrose, 0.8% agar, 2,4-dichlorophenoxyacetic acid 2,4-D and kinetin.  Maximum callus induction (94%, 92%) was observed in cotyledons and mature seeds on MS medium supplemented with 1mg/L 2,4-D, respectively. Somatic embryos were observed on mature seed explants after 17 weeks on MS medium supplemented with 5mg/L2,4-D (33%) and 0.5mg/L α-naphthaleneacetic acid(NAA) (27%). while somatic embryos were observed on cotyledon explants after 18 weeks on MS medium supplemented with 2mg/L 2,4-D (10%) and 0.5mg/L NAA (25%). The highest percentage of somatic embryogenesis (40%) was obtained with 2 mg/L 2,4-D and 0.5 mg/L NAA (30%) on shoot tip explants after 13 weeks. Regeneration of adventitious buds from callus of cotyledon and mature seeds were achieved after 8 weeks on MS medium supplemented with 0.5mg/L and 1mg/L 6-benzyladenine(BA), respectively. In vitro-regenerated plantlets with well-developed roots were successfully hardened in a greenhouse and established in soil.
Generally, auxin was found critical for induction of callus and formation of somatic embryos, while the cytokinin was essential for callus differentiation and plant regeneration.
 
Keywords
Cucumber; Cytokinin; somatic embryogenesis; cotyledon, callus, Cucumis sativus L., shoot tip
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