NEW ALTERNATIVE METHOD OF DRUMSTICK TREE (Moringa oleifera Lam.) MICROPROPAGATION. | ||||
| Journal of Productivity and Development | ||||
| Article 1, Volume 18, Issue 3, August 2013, Page 339-348 PDF (317.15 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpd.2013.42580 | ||||
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| Authors | ||||
| khaled Mazrou; Mohamed Atfi* | ||||
| Genetic Engineering and Biotechnology Research Institute (GEBRI). Sadat City University, Sadat City, Egypt. | ||||
| Abstract | ||||
| Tissue culture protocol reported in this study is an alternative method suggested to produce plantlets of drumstick tree (Moringa oleifera Lam.) with uniform genotypes for breeding, in vitro and in vivo production. The highest rate of micropropagtion will often depend not only on the selection of the most suitable explants, but also on sterilization agent and the best basal medium for that tissue. Sterilizing shoot tip explants of drumstick, using 15 % of clorex for 15 minutes showed the highest survival percentage and lowest contamination as well as sufficient browning percentage. For multiple shoot induction, the shoot tip explants of drumstick were cultured on Murashige and Skoog (MS) agar medium supplemented with different concentrations of 2-isopentinyl adenine (2iP) at (0.0,0.5,1.0 and 2.0 mg/L) alone or in combination with indol butyric acid (IBA) at ( 0.0,0.1,0.5and 1.0 mg/ L). The highest shoot multiplication rate was found in the 3rd subculture. Moreover, the highest shoot number/explant (8.8) was obtained with the medium contained 0.5mg/l 2iP with 0.1mg/ L IBA and maintained at 25±2C compared to all other treatments. The control treatment alone significantly showed the highest shoot length compared to all other treatments Low concentrations (0.5mg/L ) with (0.1ml/L IBA) were more effective in increasing the number of leaves. Plantlets grown on the control medium produced the maximum number of healthy roots. The rooted plantlets were hardened and successfully established in the soil. | ||||
| Keywords | ||||
| Moringa oleifera Lam; drumstick tree; shoot tip; sterilization; shoot multiplication | ||||
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