Some Comparative Immunohistochemical Studies on the Ductus Deferens in the Donkey (Equus asinus) and Water Buffalo Bull (Bubalus bubalis).
Alkafafy, M., Attia, H., Rashed, R., Kandiel, M. (2010). Some Comparative Immunohistochemical Studies on the Ductus Deferens in the Donkey (Equus asinus) and Water Buffalo Bull (Bubalus bubalis).. EKB Journal Management System, 3(1), 55-69. doi: 10.21608/jva.2010.42681
Mohamed Alkafafy; Hossam Attia; Reda Rashed; Mohamed Kandiel. "Some Comparative Immunohistochemical Studies on the Ductus Deferens in the Donkey (Equus asinus) and Water Buffalo Bull (Bubalus bubalis).". EKB Journal Management System, 3, 1, 2010, 55-69. doi: 10.21608/jva.2010.42681
Alkafafy, M., Attia, H., Rashed, R., Kandiel, M. (2010). 'Some Comparative Immunohistochemical Studies on the Ductus Deferens in the Donkey (Equus asinus) and Water Buffalo Bull (Bubalus bubalis).', EKB Journal Management System, 3(1), pp. 55-69. doi: 10.21608/jva.2010.42681
Alkafafy, M., Attia, H., Rashed, R., Kandiel, M. Some Comparative Immunohistochemical Studies on the Ductus Deferens in the Donkey (Equus asinus) and Water Buffalo Bull (Bubalus bubalis).. EKB Journal Management System, 2010; 3(1): 55-69. doi: 10.21608/jva.2010.42681

Some Comparative Immunohistochemical Studies on the Ductus Deferens in the Donkey (Equus asinus) and Water Buffalo Bull (Bubalus bubalis).

Journal of Veterinary Anatomy
Article 5, Volume 3, Issue 1, April 2010, Page 55-69  XML PDF (891.47 K)
Document Type: Original Article
DOI: 10.21608/jva.2010.42681
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Authors
Mohamed Alkafafy1; Hossam Attia2; Reda Rashed3; Mohamed Kandiel4
1Department of Cytology and Histology, Faculty of Veterinary Medicine, Minuofiya University, Sadat City Branch, Egypt
2Department of Histology and Cytology, Faculty of Veterinary Medicine, Benha University, Tokheh, Kaliobia, Egypt.
3Department of Anatomy and Embryology, Faculty of veterinary Medicine, Minoufiya University, Sadat City branch, Egypt.
4Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, Tokheh, Kaliobia, Egypt.
Abstract
The current work aimed to apply immu- nohistochemistry (IHC) to evaluate the differential distribution pattern of some biologically active proteins in the ductus deferens (DD) in two different animal species common in the Egyptian farms. Immunolocalization of S100, angiotensin converting enzyme (ACE), α- smooth muscle actin (α-SMA), connexin 43 (Cx43) and galactosyltransferase (GalTase) was practiced on paraffin- embedded sections of DD from clinically healthy adult five donkeys and five water buffalo bulls. The DD, in both species, was lined by a folded, high cuboidal (in donkey) or tall columnar (in buffalo) pseudostratified epithelium with stereocilia. IHC revealed that all antibodies used, displayed variable reactivities in the two species. Nerves and the periductal smooth muscles expressed a strong immunoreactivity (IR) for S100and α-SMA respectively; both in the donkey and in the buffalo. Stereocilia of principal cells showed a marked ACE- immunostaining in the donkey but not in the buffalo. Cx43-binding sites were found between the basal portions of the ductal epithelium and the subjacent interstitium; as well as among the periductal smooth muscle cells (SMCs) only in the buffalo bulls. Distinct GalTase- binding sites were found in the ductal epithelium only in the donkey. In conclusion the present findings exhibited a species-specific pattern of distribution for most of proteins under study, sug- gesting a variable functional signific- ance of the DD in the different animal species.
Keywords
donkey; Ducts deferens; Immunohistochemistry; Water buffalo
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