The Possible Antidiabetic Role of Dapagliflozin and /or Curcumin on Some Organs of Adult Male Albino Rats | ||||
| Egyptian Academic Journal of Biological Sciences, B. Zoology | ||||
| Volume 17, Issue 1, June 2025, Page 327-364 PDF (2.8 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/eajbsz.2025.431922 | ||||
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| Authors | ||||
| Fatma M. El-deep; Hemmat M. Abdelhafez; Ahkam M. El- Gendy; Rasha A. El Sayed | ||||
| Zoology and Entomology Department, Faculty of Science (Girls), Al-Azhar University, Cairo, Egypt. | ||||
| Abstract | ||||
| Background: diabetes is a prevalent endocrine metabolic disorder, particularly type 2 diabetes mellitus (T2DM), which is a serious worldwide health issue with serious complications, as diabetic nephropathy that necessitates ongoing treatment and supervision. A sodium-glucose cotransporter-2 (SGLT2) inhibitor called dapagliflozin (DAPA) is used to treat type 2 diabetes, but many diabetic drugs are costly and have adverse effects. Polyphenols like curcumin (CUR) are promising natural agents for reducing diabetic complications. Aim of the work: this study examined the potential therapeutic effects of DAPA and/or CUR in curing diabetic renal and pancreatic damage induced by a high-fat diet (HFD) combined with low-dose streptozotocin (STZ) in rats. Materials and Methods: Six groups of 36 male albino rats, each weighing between 190 and 200 g (6 rats/ group). Group I represented negative control, and group II received 80 mg/kg/day of curcumin orally. Groups III to VI underwent induction of T2DM by feeding rats with HFD for 3 weeks and injecting one dose of 40 mg/kg STZ, whereas group III was a diabetic group (positive control). However, group IV was treated with DAPA)1 mg/kg/day(, group V was treated with curcumin) 80 mg/kg/day(, and group VI was treated with a combination of DAPA and curcumin. After 30 days, kidney functions, malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), tumor necrosis factor-alpha (TNF-α), interleukin 1 beta (IL-1β), histopathological and immunohistochemical changes in renal and pancreatic tissues were evaluated. Results: the diabetic group showed elevated kidney parameters, oxidative stress, inflammation markers, and apoptosis, accompanied by a significant decrease in the antioxidant markers along with and severe renal and pancreatic tissue damage. Treatments with DAPA, CUR, or their combination significantly restored antioxidant levels (GSH, SOD), lowered MDA levels, decreased inflammatory markers (TNF-α, IL-1β) resulting in notable amelioration of renal and pancreatic tissues damage. Improved renal and pancreatic tissue structure and immunohistochemical analysis were confirmed by nearly standard structure of both organs and the reduction of caspase-3 expression, respectively. The combined DAPA/CUR treatment demonstrated superior protective effects compared to monotherapy. Conclusion: diabetes-induced complications are mitigated effectively by regulating oxidative stress, inflammation, and apoptosis, providing robust pancreatic-renal protection using combined DAPA/CUR treatment. | ||||
| Keywords | ||||
| Diabetes mellitus (DM); Curcumin (CUR); dapagliflozin (DAPA); oxidative stress; caspase-3; pancreatic-renal tissue | ||||
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