Histological and Biochemical Comparative Study Between the Effect of Quercetin and Topiramate on Induced Diabetic Peripheral Neuropathy in Adult Male Albino Rats | ||
| Egyptian Journal of Histology | ||
| Article 23, Volume 48, Issue 3, September 2025, Pages 1138-1161 PDF (14.41 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejh.2025.377584.2256 | ||
| Authors | ||
| Mai Amin Almoatasem1; azza Saleh embaby2; salwa khaled mostafa* 3; Asmaa Mahmoud Mostafa2; Fatma Elzahraa mohamed Abdellatef4 | ||
| 1Histology and Cell Biology Department, Faculty of Medicine, Beni-Suef University, | ||
| 2Histology department, Faculty of Medicine, Beni-Suef University | ||
| 3faculty of medicine benisuef university | ||
| 4Medical Histology & Cell Biology Department Faculty of Medicine-Beni-Suef University | ||
| Abstract | ||
| Introduction: Diabetic peripheral neuropathy (DPN) is a prevalent consequence for diabetes mellitus, significantly attributing to risk of amputation and mortality, if untreated. Aim of the Work: To emphasize the possible regenerative capacities of Quercetin and Topiramate against induced DPN in rat models. Material and Methods: 50 adult male albino rats were allocated into; control group (n=10) and experimental (diabetic) group (n=40) injected once by alloxan (150mg /kg/i.p). After establishing the diabetic model, rats were subdivided equally into 4 subgroups. Diabetic group, Quercetin treated diabetic group received Quercetin (100mg/Kg), Topiramate treated diabetic group received Topiramate (30mg/kg), and Combined treatment diabetic group received both Quercetin and Topiramate. Treatments were given by gastric gavage for 4 weeks then the blood samples were biochemically tested. Sciatic nerves were harvested for structural, ultrastructural and immunohistochemical assessment. H & E and toluidine were used for histological analyses and immunohistochemistry for assessing Caspase-3 and S-100 expressions and electron microscopy for ultrastructural examination. Data were analysed using computer software for morphometrical and statistical analysis. Results: The diabetic group showed a remarkable structural, biochemical and immunohistochemical changes. There was a significant decline in nerve fibres count with disturbed organization, abnormal myelin configurations, axonopathy and a significant increase and decrease in Caspase-3 and S-100 immunoreactivity. Quercetin and Topiramate treatments resulted in a partial improvement with comparable effect. Interestingly, combined therapy resulted in remarkable improvement. Conclusion: Concomitant administration of Quercetin and Topiramate have a superior therapeutic effect in a modal of a diabetic neuropathy than using either agent. | ||
| Keywords | ||
| Diabetic neuropathy; quercetin; topiramate | ||
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