Coagulase Negative Staphylococci as an emerging cause of bovine mastitis: prevalence, antimicrobial resistance and biofilm formation | ||||
| Journal of Veterinary Medical Research | ||||
| Article 1, Volume 24, Issue 1, June 2017, Page 1-11 PDF (973.79 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jvmr.2017.43256 | ||||
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| Authors | ||||
| Fawzy R. El-Seedy; Ismail A. Radwan; Walid H. Hassan; Amr Shehata | ||||
| Department of Bacteriology, Mycology and Immunology Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt | ||||
| Abstract | ||||
| "> Coagulase negative Staphylococci are the most prevalent cause of bovine subclinical mastitis. The current study were designed to study their occurrence, antibiogram and their ability to form biofilms. A total number of 95 CNS isolates were recovered from 400 lactating. S. xylosus (36.84%), S. chromogenes (12.63%), S. epidermidis (10.53%), S. saprophyticus (8.42%), S. haemolyticus (7.38%) were the most common recovered species. Disk diffusion method against 14 antimicrobials discs was used to detect their antibiogram. 100% were sensitive to Imipenem, 96.84% were sensitive to Enrofloxacin, 85.26% to Chlramphenicol and 84.21% to Vancomycin. But, 95.79% were resistant to Ampicillin, 77.9% resistant to Cefoxitin, 35.8% resistant to Cefuroxime, 32.63% resistant to Amoxycillin and 18.95% resistant to Clindamycin. Cultivation on Congo Red Agar (CRA) was carried out to detect biofilm formation. 47.37% were positive and S. epidermidis was the most biofilm positive species on CRA by the percentage of 70%. Haemolysins were studied by cultivating CNS on sheep blood agar. 25.26% were β-haemolytic, 71.57% (n=68) were γ- haemolytic and 3.15% were α- haemolytic. | ||||
| Keywords | ||||
| Coagulase Negative Staphylococci; Bovine Mastitis; Antimicrobial resistance; Biofilm | ||||
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