Genetic analysis of multidrug resistant Salmonella isolated from broiler chickens
H. Hassan, W., H. Abed, A., Thabet, A., A.M. El Nady, E. (2018). Genetic analysis of multidrug resistant Salmonella isolated from broiler chickens. EKB Journal Management System, 25(1), 121-131. doi: 10.21608/jvmr.2018.43309
Walid H. Hassan; Ahmed H. Abed; Abd El Rady Thabet; Eman A.M. El Nady. "Genetic analysis of multidrug resistant Salmonella isolated from broiler chickens". EKB Journal Management System, 25, 1, 2018, 121-131. doi: 10.21608/jvmr.2018.43309
H. Hassan, W., H. Abed, A., Thabet, A., A.M. El Nady, E. (2018). 'Genetic analysis of multidrug resistant Salmonella isolated from broiler chickens', EKB Journal Management System, 25(1), pp. 121-131. doi: 10.21608/jvmr.2018.43309
H. Hassan, W., H. Abed, A., Thabet, A., A.M. El Nady, E. Genetic analysis of multidrug resistant Salmonella isolated from broiler chickens. EKB Journal Management System, 2018; 25(1): 121-131. doi: 10.21608/jvmr.2018.43309

Genetic analysis of multidrug resistant Salmonella isolated from broiler chickens

Journal of Veterinary Medical Research
Article 12, Volume 25, Issue 1, June 2018, Page 121-131  XML PDF (841.64 K)
Document Type: Original Article
DOI: 10.21608/jvmr.2018.43309
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Authors
Walid H. Hassan1; Ahmed H. Abed1; Abd El Rady Thabet2; Eman A.M. El Nady2
1Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suef University.
2Department of Bacteriology, Animal Health Research Institute, Assiut Branch.
Abstract
"> Salmonellosis is a major problem for the poultry industry, and this problem represents a critical food safety hazard. Resistance to antimicrobial agents within nontyphoidal Salmonellae is a serious problem. The present study aimed to analyze genetically some β-lactamase resistance genes and some virulence associated genes in Salmonella isolates from broiler chicken. Five hundred samples were collected from diseased broiler chickens of different ages (3-6 weeks) from different farms in Assiut Governorate in Egypt during the period from January 2015 to December 2015. Bacteriological examination showed that 26 Salmonella isolates were recovered with a prevalence rate of 5.2% Serotyping of Salmonella isolates showed that S. Enteritidis S. Typhimurium, and S. Kentuky were identified at rates of 50%, 30.8% and 19.2%, respectively. Results of antibiogram showed that 18 Salmonella isolates (92.3%) were multidrug resistant. All isolates were
screened for the presence of 2 β-lactamase resistance genes (blaCTX and blaCMY) as well as 3 virulence genes (stn, invA and hilA) using multiplex PCR. The overall prevalences were 53.9% for blaCTX and 34.6% for blaCMY. Meanwhile, stn, invA and hilA genes were found in 96.2%, 100% and 84.7% of isolates, respectively.
Keywords
multidrug resistance; Broiler Chicken; Salmonellae; β- lactamases; stn; invA; hilA
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