A qualitative immunoassay as complementary test with tuberculin skin test for detection of tuberculosis in dairy cattle
Hamdy Hassan, W., Amin Nasr, E., Mohamed Moussa, H. (2018). A qualitative immunoassay as complementary test with tuberculin skin test for detection of tuberculosis in dairy cattle. EKB Journal Management System, 25(2), 182-190. doi: 10.21608/jvmr.2017.43317
Walid Hamdy Hassan; Essam Amin Nasr; Hassan Mohamed Moussa. "A qualitative immunoassay as complementary test with tuberculin skin test for detection of tuberculosis in dairy cattle". EKB Journal Management System, 25, 2, 2018, 182-190. doi: 10.21608/jvmr.2017.43317
Hamdy Hassan, W., Amin Nasr, E., Mohamed Moussa, H. (2018). 'A qualitative immunoassay as complementary test with tuberculin skin test for detection of tuberculosis in dairy cattle', EKB Journal Management System, 25(2), pp. 182-190. doi: 10.21608/jvmr.2017.43317
Hamdy Hassan, W., Amin Nasr, E., Mohamed Moussa, H. A qualitative immunoassay as complementary test with tuberculin skin test for detection of tuberculosis in dairy cattle. EKB Journal Management System, 2018; 25(2): 182-190. doi: 10.21608/jvmr.2017.43317

A qualitative immunoassay as complementary test with tuberculin skin test for detection of tuberculosis in dairy cattle

Journal of Veterinary Medical Research
Article 4, Volume 25, Issue 2, December 2018, Page 182-190  XML PDF (447.36 K)
Document Type: Original Article
DOI: 10.21608/jvmr.2017.43317
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Authors
Walid Hamdy Hassan1; Essam Amin Nasr2; Hassan Mohamed Moussa3
1Department of Bacteriology, Mycology and Immunology, Fac. Vet Med. Beni-Suef University, Egypt.
2Department of Tuberculosis, Bacterial Diagnostic Products Veterinary Serum and Vaccine Research Institute, Cairo, Egypt.
3General Authorities for Veterinary Services.
Abstract
Bovine tuberculosis; caused by Mycobacterium bovis,is a zoonotic diseasecausing approximately 6% of total human deaths. Its economic losses are not only a reduction of 10-20% in milk production and weight, but also infertility and condemnation of meat.Many serological tests are applied for detection of tuberculosis. ELISA test has the highest sensitivity and specificity than the other serological tests for the diagnosis of tuberculosis. Several forms of new technology were brought into the diagnostic approach to mycobacterial infection. The aim of this work was to detect bovine tuberculosis by application of different serological tests. Tuberculin skin test was applied on 2650 cattle, only 63(2.4%) were positive. Forty eight (76.2%) of the slaughtered positive animals showed visible lesions (VL) while the other 15 (23.8%) had non-visible lesions (NVL). The bacteriological examination of the 63 samples revealed isolation ofM. bovis from 47 processed samples (74.6%). The results of the immunoassay test have detected 27 out of the tuberculin positive cattle, while the ELISA has detected 34 out of the positive reactor cattle. It was concluded that immunoassay and ELISA tests act as complementary tests for tuberculin skin test especially in anergic cattle.
Keywords
ELISA; M. bovis; MOTT; tuberculin reactor; lateral flow immunoassay
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