IN VITRO EVALUATION OF ENCAPSULATED PROBIOTIC BACTERIA SUPPLEMENTATION TO RUMINANT RATIONS
Makled, A., Khorshed, M., Gouda, G., El-Garhi, M., Ebeid, H., Azzaz, H., Abdelgawad, R., Zayed, M., El-Bordeny, N. (2019). IN VITRO EVALUATION OF ENCAPSULATED PROBIOTIC BACTERIA SUPPLEMENTATION TO RUMINANT RATIONS. EKB Journal Management System, 27(1), 375-382. doi: 10.21608/ajs.2019.43550
A. A. Makled; M. M. Khorshed; G. F. Gouda; M. S. El-Garhi; H. M. Ebeid; H. H. Azzaz; R. .M.A. Abdelgawad; Mona. S. Zayed; N. E. El-Bordeny. "IN VITRO EVALUATION OF ENCAPSULATED PROBIOTIC BACTERIA SUPPLEMENTATION TO RUMINANT RATIONS". EKB Journal Management System, 27, 1, 2019, 375-382. doi: 10.21608/ajs.2019.43550
Makled, A., Khorshed, M., Gouda, G., El-Garhi, M., Ebeid, H., Azzaz, H., Abdelgawad, R., Zayed, M., El-Bordeny, N. (2019). 'IN VITRO EVALUATION OF ENCAPSULATED PROBIOTIC BACTERIA SUPPLEMENTATION TO RUMINANT RATIONS', EKB Journal Management System, 27(1), pp. 375-382. doi: 10.21608/ajs.2019.43550
Makled, A., Khorshed, M., Gouda, G., El-Garhi, M., Ebeid, H., Azzaz, H., Abdelgawad, R., Zayed, M., El-Bordeny, N. IN VITRO EVALUATION OF ENCAPSULATED PROBIOTIC BACTERIA SUPPLEMENTATION TO RUMINANT RATIONS. EKB Journal Management System, 2019; 27(1): 375-382. doi: 10.21608/ajs.2019.43550

IN VITRO EVALUATION OF ENCAPSULATED PROBIOTIC BACTERIA SUPPLEMENTATION TO RUMINANT RATIONS

Arab Universities Journal of Agricultural Sciences
Article 35, Volume 27, Issue 1, March 2019, Page 375-382  XML PDF (812.38 K)
Document Type: Original Article
DOI: 10.21608/ajs.2019.43550
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Authors
A. A. Makled1; M. M. Khorshed1; G. F. Gouda1; M. S. El-Garhi2; H. M. Ebeid3; H. H. Azzaz3; R. .M.A. Abdelgawad3; Mona. S. Zayed4; N. E. El-Bordeny5
1Animal Production Dept., Fac. of Agric., Ain Shams Univ., P.O. Box 68 Hadayek Shoubra, 11241, Cairo, Egypt.
2Animal Reproduction Research Institute, Agricultural Research Center, Giza, Egypt.
3Dairy Sciences Dept., National Research Centre, Dokki, Giza, 12311, Egypt
4Agric. Microbiology Dept., Fac. of Agric., Ain Shams Univ., P.O. Box 68 Hadayek Shoubra, 11241, Cairo, Egypt
5Animal Production Dept., Fac. of Agric., Ain Shams Univ., P.O. Box 68 Hadayek Shoubra, 11241, Cairo, Egypt
Abstract
The aim of this study was to in vitro evaluate encapsulated probiotic supplementation to ruminant rationson degradation and fermentation parameters. The ration consisted of 40% alfalfa hay and 60% concentrate feed mixture. Encapsulated and not encapsulated probiotic were supplemented with level of 106cfu/kg of the total dry matter of ration (DM) and compared with encapsulation media (Sodium Alginate, SA) and control (not supplemented ration). DM and OM degradation and total gas production as well as fermentation parameters of the incubated samples were determined after 24 h of fermentation. Significant (P<0.01) increases in in-vitro DM degradability was observed for the experimental ration supplemented with encapsulated or not encapsulated probiotics at levels (106 CFU/ kg DM) and SA treatment compared to control ration. Also, significant (P<0.05) improvement in OM degradability was recorded for the ration supplemented with not encapsulated probiotics bacteria compared to the other treatments. Moreover no significant differences were observed between the control ration and the rations supplemented with encapsulated probiotics or SA only, as well as no significant difference was recorded between the ration supplemented with encapsulated probiotics and the ration supplemented with SA only. Probiotics bacteria supple
mentation in the form of not encapsulated probiotic resulted significant increases in in vitro total gas production per sample and per g DM, OM, dDM, NDF and ADF after 24 hours incubation period compared to the other experimental rations (control, encapsulated probiotic and SA). While significant increase in total gas production per g dOM was observed for not encapsulated probiotic compared to encapsulated probiotic only. It could be concluded that, using encapsulated probiotics bacteria had no significant effect on DM degradability and may be induce decrease in gas production and fermentation parameters. 
 
Keywords
probiotics; Encapsulation; In-vitro; fermentation; Degradation
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