ISOLATION, IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF PECTINASE PRODUCING BACTERIAL ISOLATE Klebsiella oxytoca
Abdel-Aziz, N., Atallah, A., Abdel-Aal, S., Mohamed, E., Ibrahim, S., Zein Elabedeen, A. (2019). ISOLATION, IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF PECTINASE PRODUCING BACTERIAL ISOLATE Klebsiella oxytoca. EKB Journal Management System, 27(1), 553-561. doi: 10.21608/ajs.2019.43665
Nagwa. M. Abdel-Aziz; A. G. Atallah; S. Kh. Abdel-Aal; E. A. Mohamed; S. A. Ibrahim; A. Zein Elabedeen. "ISOLATION, IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF PECTINASE PRODUCING BACTERIAL ISOLATE Klebsiella oxytoca". EKB Journal Management System, 27, 1, 2019, 553-561. doi: 10.21608/ajs.2019.43665
Abdel-Aziz, N., Atallah, A., Abdel-Aal, S., Mohamed, E., Ibrahim, S., Zein Elabedeen, A. (2019). 'ISOLATION, IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF PECTINASE PRODUCING BACTERIAL ISOLATE Klebsiella oxytoca', EKB Journal Management System, 27(1), pp. 553-561. doi: 10.21608/ajs.2019.43665
Abdel-Aziz, N., Atallah, A., Abdel-Aal, S., Mohamed, E., Ibrahim, S., Zein Elabedeen, A. ISOLATION, IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF PECTINASE PRODUCING BACTERIAL ISOLATE Klebsiella oxytoca. EKB Journal Management System, 2019; 27(1): 553-561. doi: 10.21608/ajs.2019.43665

ISOLATION, IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF PECTINASE PRODUCING BACTERIAL ISOLATE Klebsiella oxytoca

Arab Universities Journal of Agricultural Sciences
Article 52, Volume 27, Issue 1, March 2019, Page 553-561  XML PDF (934.08 K)
Document Type: Original Article
DOI: 10.21608/ajs.2019.43665
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Authors
Nagwa. M. Abdel-Aziz1; A. G. Atallah1; S. Kh. Abdel-Aal1; E. A. Mohamed1; S. A. Ibrahim2; A. Zein Elabedeen2
1Genetic Microbial Dept., National Research Center, Dokki, Giza, Egypt
2Genetic Dept., Fac. of Agric., Ain Shams Univ., P.O. Box 68, Hadyek Shoubra 11241, Cairo, Egypt
Abstract
Twenty two bacteria were isolated from soil samples local. were assayed for pectinolytic activity after optimization condition for pectinase production. isolate No. 22 showed high pectinase specific activity (91.4U/ml)  on glucose supplemented medium. the identification of the isolate revealed that it belongs to the Genus klebseilla bassed on morphological, biochemical characteristic, growth and molecular level 16s rRNA indicated that isolate was 99% similarity with Klebsiella oxytoca JKo3. Therefore, it was named Klebsiella oxytoca N22.
Keywords
pectin; Pectinase; Pectinase producing bacterial Klebsiella oxytoca
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