DIRECT EMBRYOGENESIS AND INDIRECT ORGANOGENESIS OF DATE PALM (PHOENIX DACTYLIFERA L.) CV. SEWI USING IMMATURE FEMALE INFLORESCENCES
Malhat, M., El-Wakeel, H., Abd El-Hamid, A., Khalil, S., Hassan, M. (2019). DIRECT EMBRYOGENESIS AND INDIRECT ORGANOGENESIS OF DATE PALM (PHOENIX DACTYLIFERA L.) CV. SEWI USING IMMATURE FEMALE INFLORESCENCES. EKB Journal Management System, 27(1), 737-747. doi: 10.21608/ajs.2019.43693
Mervat M.H. Malhat; H. El-Wakeel; A. Abd El-Hamid; S. S. Khalil; Mona M. Hassan. "DIRECT EMBRYOGENESIS AND INDIRECT ORGANOGENESIS OF DATE PALM (PHOENIX DACTYLIFERA L.) CV. SEWI USING IMMATURE FEMALE INFLORESCENCES". EKB Journal Management System, 27, 1, 2019, 737-747. doi: 10.21608/ajs.2019.43693
Malhat, M., El-Wakeel, H., Abd El-Hamid, A., Khalil, S., Hassan, M. (2019). 'DIRECT EMBRYOGENESIS AND INDIRECT ORGANOGENESIS OF DATE PALM (PHOENIX DACTYLIFERA L.) CV. SEWI USING IMMATURE FEMALE INFLORESCENCES', EKB Journal Management System, 27(1), pp. 737-747. doi: 10.21608/ajs.2019.43693
Malhat, M., El-Wakeel, H., Abd El-Hamid, A., Khalil, S., Hassan, M. DIRECT EMBRYOGENESIS AND INDIRECT ORGANOGENESIS OF DATE PALM (PHOENIX DACTYLIFERA L.) CV. SEWI USING IMMATURE FEMALE INFLORESCENCES. EKB Journal Management System, 2019; 27(1): 737-747. doi: 10.21608/ajs.2019.43693

DIRECT EMBRYOGENESIS AND INDIRECT ORGANOGENESIS OF DATE PALM (PHOENIX DACTYLIFERA L.) CV. SEWI USING IMMATURE FEMALE INFLORESCENCES

Arab Universities Journal of Agricultural Sciences
Article 68, Volume 27, Issue 1, March 2019, Page 737-747  XML PDF (1.1 MB)
Document Type: Original Article
DOI: 10.21608/ajs.2019.43693
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Authors
Mervat M.H. Malhat1; H. El-Wakeel2; A. Abd El-Hamid2; S. S. Khalil1; Mona M. Hassan3
1Genetic Engineering Research Institute, Agric., Res. Center, Giza, Egypt
2Horticulture Dept., Fac. of Agric., Ain Shams Univ., P.O. Box 68, Hadyek Shoubra11241, Cairo, Egypt
3Central Laboratory for Date Palm Researches Development, Agric., Res-Center, Giza, Egypt
Abstract
This study was achieved at the Tissue Culture Laboratory of the Agricultural Genetic Engineering Research Institute, Giza, Egypt during the period from 2013 to 2017, Direct embryo initiation and callus formation of date palm cv. Sewi from immature female inflorescences have been achieved on modified MS medium supplemented with 4 mg l-1 Picloram plus 3 mg l-1 2 iP and 2 g l-1 PVP. Results also showed that BA at 0.5 mg l-1 produce the highest number of differentiated embryos/culture, while kinetin at 0.25 mg l-1 significantly increased the average number of adventitious shoots/culture. NAA at 1.0 mg l-1 induced the highest rooting percentage and micro-shoot length. On the other hand the best survival percentage during the acclimatization stage was observed with plantlets produced from IBA at 0.5 mg l-1 during the rooting stage.
Keywords
in Vitro; Phoenix dactylifera L; cv. Sewi; Immature Inflorescence; Direct embryogenesis; Indirect organogenesis; callus formation; Embryo formation
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