Immunity Changes in Locusta migratoria Linnaeus (Orthoptera: Acrididae) Infected by Entomopathogenic Nematode Steinernema carpocapsae (Rhabditida: Steinernematidae) | ||||
| Journal of Plant Protection and Pathology | ||||
| Article 19, Volume 9, Issue 12, December 2018, Page 877-881 PDF (334.55 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jppp.2018.44100 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Samira M. N. Abd-El Wahed  1; Nabawia M. Elhadidy2
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| 1Plant Protection Research Institute, Agric. Res. Center, Giza, Egypt | ||||
| 2Dept. of Zoology, Faculty of Science, El-Arish University, EGYPT | ||||
| Abstract | ||||
| Entomopathogenic nematode (EPNs) Steinernema carpocapsae was tested against the Locusta migratoria L., 5th instar nymphs under laboratory conditions. The mortality of nymphs maintained on three concentrations (10, 20 and 40 IJs/g) nematode-inoculated sand was particularly high. Nematode was effective, causing 100% mortality of fifth nymphal instars after third days of treatment. The impact of S. carpocapsae parasitation on L. migratoria immune defense was closely examined, after both 24-48h post treatment with LC50 of nematode the activities of phenoloxidase (PO), peroxidase (POD), α and β estrases (ESTs) and glutathione-S-transferase (GSTs) enzymes of the whole body were estimated. S. carpocapsae affect locust causing high mortality rate, which significantly increased in concentrations and time dependent manner, this effect might be because of enzyme system distraction. These results demonstrated that, the entomopathogenic nematode broke host immunity, by the changes of enzymes activities; that provide an overview about the efficiency of nematode and its effects on L. migratoria. | ||||
| Keywords | ||||
| Locusta migratoria; Steinernema carpocapsae; immunity; Phenoloxidase; Perooxidase; α and β esterases; Glutathione-S-transferase and enzymes | ||||
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