Ecofriendly spectrophotometric techniques for the determination of imeglimin hydrochloride in pharmaceutical formulations | ||
| Bulletin of Faculty of Science, Zagazig University | ||
| Article 12, Volume 2025, Issue 4, October 2025, Pages 126-136 PDF (820.27 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bfszu.2025.372235.1495 | ||
| Authors | ||
| Ayman A Gouda* 1; Ragaa elsheikh2; Asmaa S Abdelhamed2; Mohamed A. Afify2; Mona A El-Attar3; Mahmoud Almasry2 | ||
| 1Chemistry Department, Faculty of Science, Zagazig University, Zagazig, Egypt | ||
| 2Chemistry Department, Faculty of Science, Zagazig University, Zagazig, 44519, Egypt. | ||
| 3High Institute of Engineering & Technology, Tanta | ||
| Abstract | ||
| Accurate, precise, eco-friendly, sensitive, and validated spectrophotometric methods were described for determination of imeglimin hydrochloride (IMG) in pure form and pharmaceutical formulations. These methods utilize N-bromosuccinimide (NBS) as an environmentally friendly oxidizing agent alongside three specific dyes: indigocarmine (IC), rhodamine B (RB), and orange G (OG). Under acidic conditions, excess NBS is used to oxidize IMG, and the unreacted NBS is subsequently quantified through its reaction with predetermined amounts of the dyes. Absorption measurements were recorded at 610 nm for IC, 550 nm for RB, and 478 nm for OG. The analytical technique was implemented and validated by carefully examining and optimizing various factors that could potentially affect the reaction. Strong linear correlations, with correlation coefficients ranging from 0.9990 to 0.9994, were established under optimal conditions. These methods were effective within concentration ranges of 1.0–14, 1.0–12, and 1.0–16 µg/ml, respectively. The limit of detection (LOD) was determined to be 0.29 µg/ml for IC, while RB and OG exhibited an LOD of 0.30 µg/ml. The accuracy and precision of these methods were assessed through both intra-day and inter-day measurements. No significant interference was detected from common tablet excipients. | ||
| Keywords | ||
| Imeglimin hydrochloride; N-bromosuccinimide; Spectrophotometry; Method validation; Tablets | ||
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