Prevalence and Genetic Detection of L. Monocytogenes from Milk and some Milk Products
A. Abd-El Tawab, A., I. El-Hofy, F., A. Mobarez, E., Y. Tawkol, N. (2018). Prevalence and Genetic Detection of L. Monocytogenes from Milk and some Milk Products. EKB Journal Management System, 34(3), 259-266. doi: 10.21608/bvmj.2018.44750
Ashraf A. Abd-El Tawab; Fatma I. El-Hofy; Elham A. Mobarez; Nancy, Y. Tawkol. "Prevalence and Genetic Detection of L. Monocytogenes from Milk and some Milk Products". EKB Journal Management System, 34, 3, 2018, 259-266. doi: 10.21608/bvmj.2018.44750
A. Abd-El Tawab, A., I. El-Hofy, F., A. Mobarez, E., Y. Tawkol, N. (2018). 'Prevalence and Genetic Detection of L. Monocytogenes from Milk and some Milk Products', EKB Journal Management System, 34(3), pp. 259-266. doi: 10.21608/bvmj.2018.44750
A. Abd-El Tawab, A., I. El-Hofy, F., A. Mobarez, E., Y. Tawkol, N. Prevalence and Genetic Detection of L. Monocytogenes from Milk and some Milk Products. EKB Journal Management System, 2018; 34(3): 259-266. doi: 10.21608/bvmj.2018.44750

Prevalence and Genetic Detection of L. Monocytogenes from Milk and some Milk Products

Benha Veterinary Medical Journal
Article 21, Volume 34, Issue 3, August 2018, Page 259-266  XML PDF (339.26 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2018.44750
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Authors
Ashraf A. Abd-El Tawab1; Fatma I. El-Hofy2; Elham A. Mobarez3; Nancy, Y. Tawkol4
1Bacteriology, Immunology and Mycology Dep., Faculty of Vet. Med., Benha Uni.
2Bacteriology, Immunology and Mycology Dep., Faculty of Vet. Med., Benha Uni
3Animal Health Research Institute Dokki, Giza
4Veterinarian
Abstract
   A total of 200 random samples of fresh dairy milk (80), soft cheese (40), kariesh cheese (40) and ice cream (40) were collected from small retails and different shops at Qaliopia and Giza governorates during the period of October 2016 to January 2017 and transferred with minimum delay to laboratory for detection the presence of Listeria species. The bacteriological results revealed that, 5/200(2.5%) were Listeria monocytogenes (L. monocytogenes) includes 3/80 (3.75 %) from raw milk, 1/40 (2.5%) from each kariesh cheese and ice cream samples and 0/40(0%) from soft cheese. The results of Microgen TM Listeria-ID System revealed that all isolates were L. monocytogenes (99.92%). The PCR results for L. monocytogenes showed that all 16S rRNA were detected in five studied strains (100.0%) i.e., all studied strains were L. monocytogenes. 
Keywords
L. monocytogenes; Microgen TM Listeria-ID System; 16S rRNA
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