Immunohistochemical, cellular localization and expression of inhibin hormone in the buffalo (Bubalus bubalis) adenohypophysis at different ages
Attia, H., Kandiel, M., Ismail, T., Soliman, M., Nassan, M., Mansour, A. (2012). Immunohistochemical, cellular localization and expression of inhibin hormone in the buffalo (Bubalus bubalis) adenohypophysis at different ages. EKB Journal Management System, 5(2), 83-104. doi: 10.21608/jva.2012.44876
H. F. Attia; M. M. Kandiel; T. A. Ismail; M. M. Soliman; M. A. Nassan; A. A. Mansour. "Immunohistochemical, cellular localization and expression of inhibin hormone in the buffalo (Bubalus bubalis) adenohypophysis at different ages". EKB Journal Management System, 5, 2, 2012, 83-104. doi: 10.21608/jva.2012.44876
Attia, H., Kandiel, M., Ismail, T., Soliman, M., Nassan, M., Mansour, A. (2012). 'Immunohistochemical, cellular localization and expression of inhibin hormone in the buffalo (Bubalus bubalis) adenohypophysis at different ages', EKB Journal Management System, 5(2), pp. 83-104. doi: 10.21608/jva.2012.44876
Attia, H., Kandiel, M., Ismail, T., Soliman, M., Nassan, M., Mansour, A. Immunohistochemical, cellular localization and expression of inhibin hormone in the buffalo (Bubalus bubalis) adenohypophysis at different ages. EKB Journal Management System, 2012; 5(2): 83-104. doi: 10.21608/jva.2012.44876

Immunohistochemical, cellular localization and expression of inhibin hormone in the buffalo (Bubalus bubalis) adenohypophysis at different ages

Journal of Veterinary Anatomy
Article 7, Volume 5, Issue 2, October 2012, Page 83-104  XML PDF (1.45 MB)
Document Type: Original Article
DOI: 10.21608/jva.2012.44876
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Authors
H. F. Attia1; M. M. Kandiel2; T. A. Ismail3; M. M. Soliman4; M. A. Nassan5; A. A. Mansour6
1Department of Histology and Cytology, Faculty of Veterinary Medicine, Benha University, Egypt.
2Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, Egypt.
3Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Egypt & Department of Medical Laboratories and Medical Biotechnology, Faculty of Applied Medical sciences, Taif University, Turabuha branch, KSA.
4Department of Biochemistry, Faculty of Veterinary Medicine, Benha University, Egypt.
5Department of Pathology, Faculty of Veterinary Medicine, Zagazig University,
6Department of Genetics, Faculty of Agriculture, Ain Shams University, Egypt.
Abstract
 








The pituitary adenohypophysis was obtained from thirty buffaloes-cows, their age's ranges from one month to12 years. Sections of adenohypophysis tissues were immunostained for α, βa, and βb subunits of inhibin hormone. Positive immuno-staining specific for the α subunits of inhibin were detected in the cells of the follicles of the adenohypophysis in all ages. Moreover, immunostaining specific for the inhibin βa subu-








nits were strong positive at one month, weak positive at 4.5, 8 and 12 years and were negative at 8 months and 1.5 years. However, the immunostaining specific for the in- hibin βb subunits were positive at one and 8 months, 8 and 12 years and weak positive at 4.5 years and negative at 1.5 year. RT-PCR analysis revealed that both α and βb subunits are expressed in all ages except at 1.5 years old animals while βa subunit is only expressed at young age.








 
Keywords
Inhibin; Buffalo-cows; Adenohypophysis; RT-PCR; Immunohistochemistry
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