A PROTOCOL FOR MICRO-PROPAGATION OF Anthurium scherzeranum, SCHOTT PLANTS. | ||||
| Journal of Productivity and Development | ||||
| Article 3, Volume 12, Issue 2, July 2007, Page 385-399 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpd.2007.44964 | ||||
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| Author | ||||
| Azza Arafa* | ||||
| Ornamental Horticulture Department, Faculty of Agric., Cairo Univ., Egypt. | ||||
| Abstract | ||||
| This investigation was carried out in 2004 and 2005 at Plant Tissue Culture Laboratory, Agricultural Development System Project (ADSP), Ministry of Agriculture, to detect the effect of some media types, different levels of some cytokinins and growth substances on some growth and rooting parameters of Anthurium scherzeranum, Schott in vitro as well as the influence of NPK fertilization on plantlets transferred from in vitro to in vivo greenhouse conditions. The obtained results would be briefed in the following : Number of shoots and leaves were significantly raised by N & N medium, supplemented with 5.0mg/l for TDZ or BA individually. But, shoot length was significantly increased by MS medium free from TDZ or BA supplementation, whereas, fresh weight / shoot tip showed varied responses towards the two cytokinins. The MS medium supplied with TDZ at 2.0 mg/l or 5.0 mg BA/l supplemented to the N & N medium gave the heaviest fresh weight. Shoot length and fresh weight /shoot tip were significantly increased by AgNO3 at 4-5mg/l or 2.0 mg GA3 /L supplemented to MS medium, individually or in combination. Number of shoots and leaves were significantly improved by 3.0 mg /L AgNO3, but they were not affected by GA3 levels, individually; whereas 3.0 mg /L AgNO3 combined with 1.0mg GA3 /L significantly resulted the highest record of these two characters. The half strength of MS, supplemented with 0.5 mg IBA /L combination, significantly gave the best growth parameters (shoot length, number of leaves and fresh weight). While root number and length were significantly increased by 1.0 mg IBA/l added to 1/8 and 1/4 MS strengths, respectively. Plants transferred from in vitro to in vivo under greenhouse conditions showed a significant increase in number and length of shoots when fertilized with N:P:K:Mg (19:19:19:32) at rate of 4.0 g /pot, while number of leaves and flowers were significantly improved by 3.0 or 4.0 g /pot N: P: K (19:19:19), consecutively, applied in two equal doses. | ||||
| Keywords | ||||
| in Vitro; micro-propagation; thidiazuron (TDZ); benzyl adenine (BA); silver nitrate (AgNO3); Anthurium scherzeranum | ||||
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