IN VITRO PROPAGATION AND IN VIVO PRODUCTION OF Zingiber officinale,ROSC.PLANTS | ||||
| Journal of Productivity and Development | ||||
| Article 6, Volume 12, Issue 2, July 2007, Page 437-452 PDF (211.25 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpd.2007.44967 | ||||
 View on SCiNiTO | ||||
| Author | ||||
| Azza Arafa* | ||||
| Ornamental Horticulture Department, Faculty of Agric. , Cairo Univeristy, Egypt | ||||
| Abstract | ||||
| Series of experiments were undertaken at Plant Tissue Culture Laboratory, Agricultural Development System Project (ADSP), Ministry of Agriculture, during the two consecutive seasons of 2005 and 2006, to determine the influence of different sterilization materials and time, types of media, different levels of BA and NAA and sucrose concentrations on some growth and rhizome characters of common (ginger), (Zingiber officinale, Rosc.). The obtained results could be briefed in the following points: - Increasing the level of HgCl2 on NaOCl and time of immersing shoot tips, led to gradual inhibition of survival and contamination (%).- Sterilization with mercuric chloride at 0.3% level for 5 minutes followed by 1.0% sodium hypochlorite for 5 minutes also, inhibited contamination completely and 100% survival. - The best shoot number and root length were resulted by liquid media supplemented with BA at 3.0 and 1.0 mg/l, respectively. While, solid medium free from BA gave the best shoot length and number of roots.- Control treatment produced the highest length of shoot and root as well as number of roots, but number of shoots was the largest due to 0.5 mg BA + 0.5 mg NAA/l. treatment. - Raising concentrations of sucrose, reduced survival (%) gradually. The 70 and 90g/l sucrose resulted the highest formation (%) and number of rhizomes. Under greenhouse conditions, the favourable size of rhizome, as well as the levels of NPK fertilization were evaluated for growth and development of common ginger, in the two seasons. Plant height, number of leaves and fresh weights of vegetative growth and rhizomes, were increased progressively by increasing the size of the planted rhizomes (cuttings) in the greenhouse. The size of rhizomes (40 – 50g) gave growth values more than double the values of 20g rhizome. - In the greenhouse, raising N or K ratios increased gradually number of branches, leaves and roots, plant height ,root length and fresh weight of vegetative growth and rhizomes. | ||||
| Keywords | ||||
| in Vitro; Zingiber officinale; Zingiberaceae; Sterilization materials; NAA; BA; rhizome formation; in vivo; size of rhizomes; NPK; fertilization | ||||
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