APOPTOSIS, MEMORY CELL, AND T-HELPER LYMPHOCYTES BALANCE BETWEEN SEVERE AND MILD BRONCHIAL ASTHMA
Abd El-wahed, M., Ahmed, E., Mohamed, M., Said, N. (2015). APOPTOSIS, MEMORY CELL, AND T-HELPER LYMPHOCYTES BALANCE BETWEEN SEVERE AND MILD BRONCHIAL ASTHMA. EKB Journal Management System, 21(4), 1-13. doi: 10.21608/zumj.2015.4545
Maha Abd El-wahed; Ebtesam Ahmed; Mohamed Mohamed; Nora Said. "APOPTOSIS, MEMORY CELL, AND T-HELPER LYMPHOCYTES BALANCE BETWEEN SEVERE AND MILD BRONCHIAL ASTHMA". EKB Journal Management System, 21, 4, 2015, 1-13. doi: 10.21608/zumj.2015.4545
Abd El-wahed, M., Ahmed, E., Mohamed, M., Said, N. (2015). 'APOPTOSIS, MEMORY CELL, AND T-HELPER LYMPHOCYTES BALANCE BETWEEN SEVERE AND MILD BRONCHIAL ASTHMA', EKB Journal Management System, 21(4), pp. 1-13. doi: 10.21608/zumj.2015.4545
Abd El-wahed, M., Ahmed, E., Mohamed, M., Said, N. APOPTOSIS, MEMORY CELL, AND T-HELPER LYMPHOCYTES BALANCE BETWEEN SEVERE AND MILD BRONCHIAL ASTHMA. EKB Journal Management System, 2015; 21(4): 1-13. doi: 10.21608/zumj.2015.4545

APOPTOSIS, MEMORY CELL, AND T-HELPER LYMPHOCYTES BALANCE BETWEEN SEVERE AND MILD BRONCHIAL ASTHMA

Zagazig University Medical Journal
Article 6, Volume 21, Issue 4, July 2015, Page 1-13  XML PDF (1.16 MB)
Document Type: Original Article
DOI: 10.21608/zumj.2015.4545
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Authors
Maha Abd El-wahed1; Ebtesam Ahmed1; Mohamed Mohamed; Nora Said2
1Professor of clinical pathology, faculty of medicine, zagazig university,
2Assistant lecturer of clinical pathology, faculty of medicine zagazig university.
Abstract
Background: Bronchial asthma is a characterized by chronic inflammation with reversible airway obstruction and airway hyper-reactivity. In order to choose the most appropriate line of therapy we need to understand the sequence of inflammatory reaction at every stage of asthma.
Aim of the work: we aimed to study apoptosis tendency, CD4+/CD8+ ratio, memory /naïve , and T- helper 1 and 2 cytokines in vitro synthesis from peripheral blood lymphocytes (PBL) in mild persistent asthma and severe persistent asthma during acute exacerbation attacks. Patients and methods: This study comprised 50 participants divided into three groups: Group I: 10 healthy volunteers, Group II: 20 patients of mild persistent asthma, Group III: 20 patients of severe persistent asthma. All members of this study were subjected to: Immunophenotyping by flow-cytometry. Lymphocyte populations were analyzed by using CD3+CD4+ (T helper cells), CD3+ CD8+ (T cytotoxic cells), CD4+/CD8+ ratio, BCL2, CD45RO+ (memory T cells), and CD45RA+ (naïve T cells) markers with calculation of CD45RO+ / CD45RA+ ratio and Th2/Th1 cytokines profile, in the form of IL-4/IFN-γ ratio by Enzyme linked immunossorbant assay (ELISA). Results: We found an elevation in CD45RO and CD45RO+ / CD45RA+ ratio of T lymphocytes in severe than in mild asthmatics. Bcl-2 was elevated in severe asthma in relation to mild asthma and healthy group. While, no significant difference could be detected in CD4+/CD8+ ratio among the three groups. IL-4 and IL-4/ IFN-γ ratio were increased in the mild and severe groups compared to the control group. Conclusion: The immune reactions differs greatly according to the severity of asthma, with more memory T cells, longer life span of PBL and a greater role of Th1 cytokines.
Keywords
Asthma; TH1; Th2; IL-4; IFN-γ
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